Effects of electrical field stimulation on the proliferation and migration of Schwann cells
10.3969/j.issn.1005-202X.2025.02.015
- VernacularTitle:电场刺激对雪旺细胞增殖和迁移的影响
- Author:
Jingtian QI
1
;
Yongping YE
;
Yongjun XU
;
Qingsong SHENG
;
Longyu CAI
;
Jianwei HU
;
Yongguang ZHANG
Author Information
1. 福建医科大学福总临床医学院/厦门大学附属东方医院(第九〇〇医院)骨科,福建 福州 350025
- Publication Type:Journal Article
- Keywords:
electrical field stimulation;
electrotaxis chamber;
Schwann cell;
migration;
proliferation;
epithelial-mesenchymal transition
- From:
Chinese Journal of Medical Physics
2025;42(2):240-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an electrical field(EF)stimulation model for Schwann cells(SCs),and to provide a basis for exploring the mechanisms of EF stimulation in promoting proliferation,migration and epithelial-to-mesenchymal transition of SCs.Methods A YC-3 bipolar programmable electrical stimulator and an electrotaxis chamber were used to construct an EF stimulation system to stimulate SCs.In the study,SCs were divided into control group(Ctrl)receiving no EF stimulation and EF group stimulated by continuous constant-voltage EF(100 mV/mm,3 h).The effects of EF stimulation on the proliferation and migration of SCs were analyzed using CCK-8 assay,and wound healing assay+Transwell assay,separately;and its effect on SCs adhesion was observed by analyzing the expressions of E-cadherin and N-cadherin using Western Blot.Results The CCK-8 assay results suggested that the absorbance at 450 nm was significantly higher in EF group than in Ctrl group(P<0.05).The results of wound healing assay+Transwell assay revealed that EF group had higher cell migration efficiency than Ctrl group(P<0.05).Western Blot results showed decreased E-cadherin expression and increased N-cadherin expression in EF group as compared with Ctrl group(P<0.05).Conclusion The improved EF stimulation system for SCs is operable.EF stimulation can promote the proliferation and migration of SCs.The decreased E-cadherin expression and increased N-cadherin expression may be related to the occurrence of epithelial-to-mesenchymal transition in SCs after EF stimulation.
