Effects of immunosuppressant cyclophosphamide on"extrapulmonary trans-mission"in mouse model of influenza virus pneumonia
10.3969/j.issn.1000-484X.2025.02.027
- VernacularTitle:免疫抑制剂环磷酰胺对流感病毒性肺炎小鼠模型"肺外传变"的影响
- Author:
Cheng ZHAO
1
;
Xianggang ZHANG
;
Xiaoqi WANG
;
Jue HU
;
Chang LIU
;
Fangguo LU
Author Information
1. 湖南中医药大学中西医结合学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
Immunosuppression;
Cyclophosphamide;
Influenza virus pneumonia;
Extrapulmonary transmission
- From:
Chinese Journal of Immunology
2025;41(2):418-423,432
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe effect of immunosuppressant cyclophosphamide on"extrapulmonary transmission"of mouse model of influenza virus pneumonia.Methods:BALB/c mice were randomly divided into normal group,virus group,cyclophospha-mide group,virus+cyclophosphamide group.Cyclophosphamide group and virus+cyclophosphamide group were intraperitoneally injected with cyclophosphamide once,24 h afterwards,virus group and virus+cyclophosphamide group were administered nasal influ-enza virus to establish influenza virus infection model,and 3,5,7 days after nasal instillation,lung index,heart index,liver index,spleen index and kidney index were measured by conventional methods;pathological changes of lung tissue,heart tissue,liver tissue,spleen tissue and kidney tissue were observed by HE staining;ELISA was used to detect levels of serum inflammatory cytokines IL-1β,IL-6,TNF-α;RT-qPCR was used to detect influenza virus load and expressions of inflammatory factors IL-1β,IL-6,TNF-α in each tissue.Results:Compared with normal group and virus group,lung index and heart index of mice in virus+cyclophosphamide group were significantly increased(P<0.05 or P<0.01);spleen index was significantly decreased(P<0.05 or P<0.01);lung tissue,heart tissue,liver tissue and spleen tissue showed different degrees of pathological damage;serum inflammatory factor IL-6 level was increased significantly(P<0.05 or P<0.01);lung tissue,heart tissue,liver tissue,spleen tissue and mRNA levels of IAV NP in kidney tissue were significantly increased(P<0.05 or P<0.01);mRNA levels of IL-1β,IL-6 and TNF-α in lung and heart tissues were significantly increased(P<0.05 or P<0.01).Conclusion:Immunosuppressant cyclophosphamide can cause damage to extrapul-monary tissues and organs in a mouse model of influenza virus pneumonia,among which heart damage is the most serious.Cyclophos-phamide is beneficial to establish model of"extrapulmonary transmission"of influenza virus pneumonia.
