Effects of exposure to di-n-butyl phthalate on pregnancy outcome in infertile women and early embryo development in mice
10.3760/cma.j.cn101441-20200510-00272
- VernacularTitle:邻苯二甲酸二丁酯暴露对不孕女性助孕结局及小鼠早期胚胎发育影响的研究
- Author:
Helong ZHANG
1
;
Chenchen CUI
1
;
Lei LI
1
;
Huanhuan CHEN
1
;
Lei ZHANG
1
;
Hua ZHAO
1
Author Information
1. 河南省人民医院生殖医学中心，郑州 450003
- Publication Type:Journal Article
- Keywords:
Phthalate esters;
Di-n-butyl phthalate;
Mono-n-butyl phthalate;
In exposure;
Fertilization in vitro;
Embryo transfer
- From:
Chinese Journal of Reproduction and Contraception
2021;41(9):805-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of di-n-butyl phthalate (DBP) exposure levels on early reproductive outcomes in vivo in infertile women, and its influence on early embryonic development of DBP exposed mice. Methods:This study population sampling selected 112 patients according to the tail number of the medical record number who undergwent in vitro fertilization-embryo transfer (IVF-ET) treatment for tubal factor infertility from February to October 2019 in Reproductive Medicine Center of Henan Province People's Hospital. The concentration of DBP metabolite mono-n-butyl phthalate (MBP) in patients' urine was determined by HPLC tandem mass spectrometry. Multiple regression model analysis was used to analyze the relationship between urine level of MBP and early reproductive outcomes in patients with infertility. At the same time, 40 3-week-old clean grade ICR infected female mice were randomly divided into control (corn oil) group and low-, middle- and high-dose DBP infected (300 mg/kg, 600 mg/kg, 1200 mg/kg) groups, with 10 mice in each group. Fertilized eggs were collected from female ovulation induction and non-infected male mice in each group, and the differences of embryo cleavage rate, blastocyst formation rate and landing point were compared. Results:After controlling for the mixed factors, the concentration of MBP was negatively correlated with the fertilization rate, the number of good-quality embryos, the number of blastocyst formation and the rate of blastocyst formation as the urine level increased ( P=0.01, P=0.04, P=0.03, P=0.05). There were statistically significant differences in cleavage rate between the low-dose group and the middle-dose group [58.38% (115/197) vs. 43.42% (99/228), P=0.002], between the low-dose group and the high-dose group [58.38% (115/197) vs. 36.60% (71/194), P<0.001], and no statistically significant difference was observed between the middle-dose group and the high-dose group ( P>0.05). There was a statistical difference in blastocyst formation rate between the low-dose group and the high-dose group [54.78% (63/115) vs. 39.44% (28/71), P=0.042], and no statistical difference was observed between the other two groups ( P>0.05). There was no statistically significant difference in implantation sites among the three groups of mice exposed to DBP ( P>0.05). Conclusion:There was a correlation between DBP exposure and clinical outcomes in infertile women, and DBP exposure caused toxic damage to early embryonic development in mice.
