Effect of macrophage polarization on PI3K/Akt/mTOR signaling pathway in vascular smooth muscle cells
10.3969/j.issn.1000-484X.2025.02.010
- VernacularTitle:巨噬细胞极化对血管平滑肌细胞PI3K/Akt/mTOR信号通路的影响
- Author:
Ying FENG
1
;
Yan ZHANG
1
;
Jie LEI
1
;
Juan LIU
1
;
Yong FANG
1
;
Liqun HE
1
Author Information
1. 武汉市第一医院心内科,武汉 430000
- Publication Type:Journal Article
- Keywords:
Macrophage polarization;
Atherosclerosis;
PI3K/Akt/mTOR signaling pathway
- From:
Chinese Journal of Immunology
2025;41(2):315-319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of macrophage polarization on PI3K/Akt/mTOR signaling pathway and inflammatory response of vascular smooth muscle cells.Methods:THP-1 cells were induced to become macrophages by phaboate,then treated with LPS and IFN-γ,IL-4 and IL-13 for 48 h,and cultured with fresh medium without serum for 24 h.Supernatant was used as conditioned medium.Vascular smooth muscle cells were divided into control group,M0 medium group,M1 medium group and M2 medium group.CCK-8 was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,ELISA was used to detect expressions of inflammatory cytokines IL-1α,IL-6 and TGF-β in supernatant of cells,and mRNA and phosphorylated protein expressions of PI3K,Akt and mTOR in vascular smooth muscle cells were detected by RT-qPCR and Western blot.Results:Compared with control group,cell proliferation ability and TGF-β level in supernatant of M0 medium group were significantly decreased(P<0.01),apoptosis rate,IL-1α and IL-6 levels in cell supernatant,mRNA and protein phosphorylation levels of PI3K,Akt and mTOR in cells were significantly increased(P<0.01).Compared with M0 medium group,cell proliferation ability and TGF-β level in supernatant of M1 medium group were significantly decreased(P<0.05),apoptosis rate,IL-1α and IL-6 levels in cell supernatant,mRNA and protein phosphorylation levels of PI3K,Akt and mTOR in cells were significantly increased(P<0.01),the trend was opposite in M2 medium group(P<0.05).Conclusion:Macrophage polarization can regulate expressions of inflammatory cytokines by regulating PI3K/Akt/mTOR signaling pathway,and participate in inflammatory response in atherosclerosis.
