Effects of alveolar macrophages on allergic airway inflammation induced by house dust mites
10.3969/j.issn.1000-484X.2025.02.004
- VernacularTitle:肺泡巨噬细胞对屋尘螨诱导的过敏性气道炎症的影响
- Author:
Baihe CHENG
1
;
Wenting YANG
;
Fei HOU
;
Li TANG
Author Information
1. 青岛大学基础医学院,青岛 266071;军事科学院军事医学研究院生命组学研究所,蛋白质组学国家重点实验室,北京 102206
- Publication Type:Journal Article
- Keywords:
House dust mites;
Allergic airway inflammation;
Alveolar macrophages;
Eosinophil;
Group 2 innate lymphoid cells
- From:
Chinese Journal of Immunology
2025;41(2):276-280,286
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of immune cells in allergic airway inflammation induced by house dust mites(HDM)in the mouse model,and investigate the effect of alveolar macrophages(AM)on allergic airway inflammation.Methods:Al-lergic airway inflammation was induced by intratracheal injection of HDM,and AM were specifically depleted by intratracheal injec-tion of chlorophosphonate liposomes(CL)before HDM treatment;the immune cells of mouse lung were isolated by heart perfusion.The immune cells of mouse alveolar lavage fluid were isolated by tracheal lavage.The level of type Ⅱ cytokines in alveolar lavage fluid was determined by ELISA.The changes of immune cells in mouse lung tissue and alveolar lavage fluid were detected by flow cytome-try;HE staining and PAS staining were performed to observe the infiltration of inflammatory cell,goblet cell proliferation and mucus secretion in the lung sections after HDM treatment.Results:The effector cells of type Ⅱ immune response,such as group 2 innate lymphoid cells(ILC2)and eosinophils,were significantly increased in allergic airway inflammation induced by HDM in mice.Histo-logical analysis also revealed substantial inflammatory cell infiltration,goblet cells proliferation and mucus secretion in lung tissue.The depletion of AM alleviated HDM induced type Ⅱ immune response,but promoted the infiltration of neutrophils and aggravated the pathological injury of the lung.Conclusion:AM plays an important role in HDM induced allergic airway inflammation.
