Association between modelling cycle and antioxidant pathways in rat models of non-alcoholic fatty liver disease
10.3969/j.issn.1000-484X.2025.02.001
- VernacularTitle:非酒精性脂肪性肝病大鼠模型的造模周期与抗氧化途径的关联研究
- Author:
Guoguo ZHI
1
;
Bingjie SHAO
1
;
Yiyuan FENG
1
;
Sha ZHU
1
;
Jie MU
1
;
Dong WANG
1
Author Information
1. 成都中医药大学基础医学院,成都 611137
- Publication Type:Journal Article
- Keywords:
Nonalcoholic fatty liver disease(NAFLD);
Choline-deficient high-fat diet(CDHFD);
Animal models;
Antioxi-dant;
Nrf2 signaling pathway
- From:
Chinese Journal of Immunology
2025;41(2):257-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a rat model of non-alcoholic fatty liver disease(NAFLD)by choline-deficient high fat diet(CDHFD),and to observe the association between feeding cycle and antioxidant pathway.Methods:The study lasted 16 weeks and was divided into 4 cycles.Detection of pathological changes and expression of antioxidant enzymes in rats liver in different cycles.Results:The early stage of liver steatosis and inflammation in model rats was 2～4 weeks,non-alcoholic steatohepatitis(NASH)stage was 4～8 weeks,and liver fibrosis progression was 8～16 weeks.Mechanistic studies had shown that the expressions of antioxidant enzymes Nrf2,SOD and GSH-Px in the liver of NAFLD rats gradually decreased with the extension of the feeding cycle.Conclusion:Different modeling cycles can successfully induce the pathological changes of steatosis,inflammation and liver fibrosis in rat liver,and the pathological changes are time-dependent with the expressions of antioxidant enzymes.
