Separation,purification and immunomodulatory activity of polysaccharides from Polygonatum filipes
10.3969/j.issn.1000-484X.2025.01.027
- VernacularTitle:黄精多糖的分离纯化及其免疫调节活性研究
- Author:
Yunyun SUN
1
;
Ming ZHAO
1
;
Shiyue QIN
1
;
Sichun ZHENG
1
;
Haoxiang ZHANG
1
;
Xiaodan MA
1
;
Xiaoliang LI
1
;
Wei XU
1
Author Information
1. 浙江大学动物科学学院,杭州 310058
- Publication Type:Journal Article
- Keywords:
Polygonatum filipes;
Polysaccharides;
Separation and purification;
Macrophage;
Immunomodulation;
Signaling pathways
- From:
Chinese Journal of Immunology
2025;41(1):182-188,194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To separate and purify the polysaccharides from Polygonatum filipes,characterize their primary structure and investigate the immunomodulatory effects on RAW264.7 macrophages.Methods:Crude polysaccharides from Polygonatum filipes were extracted by ultrasound assisted method,then Polygonatum filipes polysaccharides(CSPFPs)were obtained after elimination of the proteins with combined papain-Sevag method.The total sugar content was determined by phenol-sulfuric acid method.Structures of CSPFPs were analyzed by fourier transform infrared spectroscopy(FT-IR),high performance gel permeation chromatography(HPGPC)and high performance liquid chromatography(HPLC).Effects of CSPFPs on cell viability,pinocytic activity,TNF-α secretion,MAPK and NF-κB signaling pathways of RAW264.7 cells were explored by MTT,Neutral red,ELISA and Western blot,respectively.Results:Extraction rate of CSPFPs by ultrasound-assisted method was 41.61%,which contained total sugar content of 94.00%.CSPFPs with Mw of 3 125 Da was composed of arabinose(1.85%),galactose(6.14%),glucose(56.41%)and mannose(35.60%).The in vitro experiments showed that CSPFPs were non-cytotoxic and enhanced the pinocytic activity,TNF-α secretion and phosphorylation levels of p38,ERK,JNK,p65,IκB and IKK,indicating the activation of MAPK and NF-κB signaling pathways under the concentra-tion of 2.5～200 μg/ml.Conclusion:The ultrasound-assisted method can efficiently isolate CSPFPs with immunomodulatory activity,which provides basic data for the development and application of CSPFPs as an immunostimulant.
