Mechanism of Dunhuang Ruicao ointment in treatment of frostbite based on network pharmacology and experimental verification
10.3969/j.issn.1000-484X.2025.01.020
- VernacularTitle:基于网络药理学及实验验证探讨敦煌芮草膏干预冻伤的作用机制
- Author:
Li ZHANG
1
;
Xingyao LIN
1
;
Yun SHANG
1
;
Qiang WANG
1
;
Liangliang LI
1
;
Shuang LI
1
;
Xianqin DU
1
Author Information
1. 甘肃中医药大学,兰州 730000
- Publication Type:Journal Article
- Keywords:
Dunhuang Ruicao ointment;
Network pharmacology;
Animal verification;
Toll-like receptor signaling pathway
- From:
Chinese Journal of Immunology
2025;41(1):136-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To verify mechanism of Dunhuang Ruicao ointment in frostbite treatment through network pharmacology and experiment.Methods:Targets of Dunhuang Ruicao ointment in intervention of frostbite was screened through network pharmacology.Dunhuang Ruicao ointment was used to treat rat model of frostbite.Effect of drug was observed and results of network pharmacological enrichment analysis were verified.Results:A total of 239 active drug components,186 disease targets and 36 common targets were screened.KEGG pathway enrichment screened out 96 pathways,mainly enriched in Toll-like receptor signaling pathway,IL-17 signaling pathway,tumor necrosis factor signaling pathway,AGE-RAGE signaling pathway,Th17 cell differentiation pathway,suggesting Toll-like receptor signaling pathway may play an important role in treatment of frostbite by Dunhuang Ruicao ointment.Animal experiments showed that compared with model group,skin frostbite of rats treated with different doses of Dunhuang Ruicao ointment group was significantly improved,wound healing rate was increased,swelling rate was decreased,wound tissue epithelialization was obvious,inflammatory infiltration was reduced,angiogenesis was increased,and collagen fiber content was increased.mRNA and protein expressions of TLR4,MyD88 and NF-κB p65 in local skin lesions were decreased to different degrees,and middle-dose group had the best effect.Conclusion:Dunhuang Ruicao ointment in treatment of frostbite has characteristics of multiple components,multiple targets,multiple pathways synergy,probably playing a role in treatment of frostbite by inhibiting TLR4/MyD88/NF-κB signaling path-way activated to reduce frostbite rats serum proinflammatory media release,reduce inflammation,improve rate of tissue remodeling,promote healing of frostbite,providing a theoretical basis for further study of complex mechanism of Dunhuang Ruicao ointment in treatment of frostbite.
