Influences of miR-16-5p on neuronal apoptosis in rats with intracerebral hemorrhage by regulating Hippo/YAP pathway
10.3969/j.issn.1000-484X.2025.02.013
- VernacularTitle:miR-16-5p通过调控Hippo/YAP通路对脑出血大鼠神经元凋亡的影响
- Author:
Sheng ZHAO
1
;
Hua YANG
;
Zhao WU
;
Dong JIANG
Author Information
1. 贵州医科大学临床学院,贵阳 550001;贵州医科大学附属医院金阳医院,贵阳 550001
- Publication Type:Journal Article
- Keywords:
Intracerebral hemorrhage;
miR-16-5p;
Hippo/Yes-associated protein pathway;
Neurons;
Apoptosis
- From:
Chinese Journal of Immunology
2025;41(2):336-341,350
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate influences of miR-16-5p on neuronal apoptosis in rats with intracerebral hemorrhage(ICH)by regulating Hippo/Yes-associated protein(YAP)pathway.Methods:Rats were randomly separated into normal control(NC)group,ICH model(Model)group,antogamir negative control(antogamir NC)group,miR-16-5p antagomir group,miR-16-5p antagomir+cucurbitacin B(Hippo pathway activator)group,miR-16-5p antagomir+verteporfin(YAP inhibitor)group,with 18 per group.Except for NC group,rats in other groups were injected with collagenase Ⅶ into right caudate nucleus to construct ICH model,and neurological function scores were detected after treatment;qRT-PCR was performed to detect miR-16-5p expression;dry-wet weight method was performed to measure water content of brain tissue;HE staining was used to detect pathological changes of brain tissue around hematoma;kits was used to measure levels of oxidative stress indicators superoxide dismutase(SOD),malondialdehyde(MDA),inflammatory factors IL-6 and TNF-α;TUNEL method was performed to measure nerve cell apoptosis;Western blot was performed to measure Bim,Bcl2-associated X protein(Bax),YAP1,phosphorylated c-Jun amino-terminal kinase(p-JNK)protein expressions.Results:Compared with NC group,Model group exhibited severe brain tissue damage around hematoma,increased neuro-logical function score,miR-16-5p expression,brain tissue water content,MDA,IL-6 and TNF-α levels,neuronal apoptosis rate,protein expressions of Bim,Bax and p-JNK were increased,SOD level and YAP1 protein expression were decreased.miR-16-5p antagomir had significant improvement effects on above changes in ICH rats.Both cucurbitacin B and verteporfin weakened brain protective effect of miR-16-5p antagomir on ICH rats.Conclusion:Protective effect of down-regulation of miR-16-5p in ICH rats may be related to inhibition of Hippo pathway and YAP activation.
