GB promotes phagocytosis of myelin debris by astrocytes and mechanism discussion
10.3969/j.issn.1000-484X.2025.01.003
- VernacularTitle:银杏内酯B促进星形胶质细胞吞噬髓鞘碎片及其机制探讨
- Author:
Xiaohui LI
1
;
Kexin LIU
;
Lijuan SONG
;
Jiezhong YU
;
Dong MA
;
Jianjun HUANG
;
Qing WANG
;
Cungen MA
Author Information
1. 山西中医药大学神经生物学研究中心/国家中医药管理局多发性硬化益气活血重点研究室,晋中 030619
- Publication Type:Journal Article
- Keywords:
Ginkolactone B;
Astrocytes;
Myelin debris;
Apoptosis
- From:
Chinese Journal of Immunology
2025;41(1):19-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ginkgolide B(GB)on astrocyte(AST)phagocytosis of myelin debris,and to investigate the mechanism of this functional therapy to demyelination by targeting AST.Methods:In vitro culture of AST,and divided AST into three groups:Control group,Myelin debris(Debris)group and Debris+GB group,incubed them in a constant temperature CO2 cell culture incubator for 24 hours,and then detected relevant indicators to observe the effect of GB on AST on phagocytic myelin debris.Results:Compared to the phagocytosis of myelin debris by primary AST,GB could effectively promote the phagocytosis by AST and show enhanced ABCA-1 expression(both P<0.05).Phagocytosis of myelin debris had no effect on the secretion of inflammatory cytokines by in vitro cultured AST.Debris+GB increased the expressions of neurotrophic CNTF and B-FGF compared to Debris(both P<0.05).Furthermore,Debris+GB decreased Bax and Caspase-3,while increased Bcl-2 expression(all P<0.05).Conclusion:GB can promote the phagocytosis of myelin debris by AST,which may be related to the upregulation of ABCA-1.Meanwhile,phagocytosis of myelin debris by AST increases the expression of the neurotrophic factors and the inhibits the apoptosis of AST themselves.
