Mycoplasma and ureaplasma infection and drug sensitivity analysis on 850 sperm cryopreservation patients in Sichuan area
10.3760/cma.j.cn101441-20200424-00243
- VernacularTitle:四川地区850例自体精液保存患者支原体感染现状及药敏分析
- Author:
Yang XIAN
1
;
Bo LIU
;
Shasha LIU
;
Wenrui ZHAO
;
Bin ZHOU
;
Xiao LIU
;
Min JIANG
;
Fuping LI
Author Information
1. 四川大学华西第二医院人类精子库/出生缺陷与妇幼相关疾病教育部重点实验室，成都 610041
- Publication Type:Journal Article
- Keywords:
Mycoplasma and ureaplasma infections;
Antimicrobial susceptibility test;
Sperm cryopreservation
- From:
Chinese Journal of Reproduction and Contraception
2021;41(6):551-556
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the mycoplasma and ureaplasma infection and drug sensitivity analysis on sperm cryopreservation patients in Sichuan area, in order to provide reference for clinical rational use of the drug.Methods:A cross-sectional study was conducted from November 2013 to March 2020, 850 patients who came to human sperm bank of West China Second University Hospital, Sichuan University for sperm cryopreservation participated in this study. Sensitivity analysis of the mycoplasma and ureaplasma were performed. All the patients were classified into 5 groups to compare the mycoplasma and ureaplasma statues according to the age: 13-20 years old, 21-30 years old, 31-40 years old, 41-50 years old, >50 years old; according to the marital status of the patients, the patients were divided into married and unmarried to compare the mycoplasma and ureaplasma infection.Results:1) There were 167 cases of the mycoplasma and ureaplasma infection in sperm cryopreservation patients, the positive rate was 19.65%. The positive rate of mycoplasma and ureaplasma infection in all age groups had a significant difference ( P<0.001), among which the group of >50 years old had the highest positive rate [35.29% (6/17)], followed by 31-40 years old group [26.23% (80/307)], and the 13-20 years old group had the lowest positive rate of mycoplasma and ureaplasma infection [2.86% (2/70)]. The mycoplasma and ureaplasma infection rate of married people [23.51% (91/387)] was significantly higher than that of unmarried people [16.41% (76/463)] ( P=0.009). 2) The antimicrobial susceptibility test (AST) in 167 mycoplasma and ureaplasma-positive patients showed that the most sensitive drugs for mycoplasma and ureaplasma were josamycin, followed by roxithromycin, azithromycin, doxycycline and clarithromycin; the most resistance drugs for mycoplasma and ureaplasma were ciprofloxacin, followed by sparfloxacin, levofloxacin and oxygen ofloxacin. AST showed that the most resistance drugs for mycoplasma and ureaplasma were quinolone antimicrobials, and there was significant differences in the resistance of quinolone antimicrobials in different years ( P=0.001). Conclusion:The mycoplasma and ureaplasma infection rate of sperm cryopreservation patient was at a high level, married patients and reproductive-age patients had a higher rate of mycoplasma and ureaplasma infection. It is recommended that patients with positive mycoplasma and ureaplasma infection should be treated before self-sperm preservation, and the semen should be frozen after mycoplasma and ureaplasma infection become negative. During clinical treatment, antibiotics should be selected according to drug sensitivity experiments to avoid drug resistance.
