Bioinformatics and experimental validation reveal the core genes of neutrophil extracellular traps in chronic obstructive pulmonary disease
10.3969/j.issn.1000-484X.2025.01.005
- VernacularTitle:生物信息学和实验验证揭示中性粒细胞诱捕网在慢性阻塞性肺疾病中的核心基因
- Author:
Ting XUE
1
;
Leting ZHENG
;
Fei DONG
;
Xiaoning ZHONG
Author Information
1. 广西医科大学第一附属医院呼吸与危重症医学科,南宁 530021
- Publication Type:Journal Article
- Keywords:
COPD;
DEGs;
NETs;
Bioinformatics
- From:
Chinese Journal of Immunology
2025;41(1):32-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Bioinformatics and experimental validation reveal the core genes of neutrophil extracellular traps(NETs)in chronic obstructive pulmonary disease(COPD).Methods:Chip data GSE8545,GSE10006,GSE11906,GSE19407 and GSE20257 were downloaded from GEO database,and the common differential expression genes(DEGs)of the first 4 datas were ob-tained through intersection and enrichment analysis were performed.Neutrophil extracellular traps differential expression genes(NETs-DEGs)were obtained by overlapping the NETs-related genes and the common DEGs,and GSE20257 was used to verify the NETs-DEGs and evaluate the diagnostic efficacy,and enrichment analysis was performed and mRNA expression level was verified in lung tissue of mice emphysema model.Results:A total of 149 common DEGs were acquired.DO analysis mainly concentrated in lung disease and COPD.GO functional annotation mainly involved in olefin metabolism,cytohormone metabolism,endoplasmic reticulum lumen,signaling receptors and oxidative stress activities.KEGG enrichment analysis mainly involved in cytochrome P450 metabo-lism,chemical carcinogenic reactive oxygen species,steroid hormone biosynthesis and phagosome,etc.GSE20257 dataset verifica-tion showed that NETs-DEGs TLR7 and CYP4F3 were up-regulated,and AUC values were 0.750 and 0.947.GO and KEGG mainly focused on fat-soluble vitamin catabolic signaling pathways,phagosomes,arachidonic acid monooxygenase activity,pattern recogni-tion receptors,arachidonic acid metabolism,Toll-like receptor signaling pathways and formation of NETs.Compared with air exposure group,mRNA expression levels of TLR7 and CYP4F3 in lung tissue of emphysema mice induced by cigarette smoke exposure were sig-nificantly increased(P<0.05).Conclusion:TLR7 and CYP4F3 may be the core genes in formation and release of NETs during the pathogenesis of COPD,which are expected to become the targets of immunotherapy for COPD.
