Mechanism of rosmarinic acid regulating NF-κB/NLRP3 signaling pathway to alleviate acute gouty arthritis in rats
10.3969/j.issn.1000-484X.2025.01.004
- VernacularTitle:迷迭香酸调节NF-κB/NLRP3信号通路减轻大鼠急性痛风性关节炎的机制研究
- Author:
Lihuan WANG
1
;
Yijie LI
;
Huan YANG
;
Jiarong LIANG
;
Bin YE
Author Information
1. 云南中医药大学针灸推拿康复学院,昆明 650500;云南中医药大学中药学院暨云南省南药可持续利用研究重点实验室,昆明 650500
- Publication Type:Journal Article
- Keywords:
Rosmarinic acid;
Acute gouty arthritis;
Inflammation;
NF-κB/NLRP3 signaling pathway;
Monomer of traditional Chinese medicine
- From:
Chinese Journal of Immunology
2025;41(1):25-31,38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-inflammatory action and mechanism of action of rosmarinic acid(RA),a Chi-nese monomeric herbal compound based on the NF-κB/NLRP3 pathway in a rat model of acute gouty arthritis(AGA).Methods:Forty healthy male SD rats were randomly assigned to five groups,and the model and drug administration groups were established a rat model of AGA by injecting a suspension of sodium urate crystals(MSU)into the joint cavity of the right paw of the rats.The degree of ankle joint swelling was measured using vernier calipers and stained with hematoxylin-eosin(HE).The inflammatory infiltration of the ankle joint and chondrocyte shedding was observed by staining with Safranine O-fast green(SOFG)and Mankin's score.The levels of IL-6,IL-1β and TNF-α in the right ankle joint of rats were determined by ELISA and the expression of NF-κB/NLRP3 pathway-associated proteins in the ankle joint were measured by Western blot.Results:Injection of MSU suspension into the ankle successfully induced AGA in rats.Compared with control group,the rats in model group showed significantly increased ankle swelling(P<0.01),greater in-flammatory infiltration,significant chondrocyte detachment,significantly increased secretion of IL-1β,IL-6 and TNF-α(P<0.01)and increased expressions of NF-κB/NLRP3 pathway-related proteins.Compared with model group,RA showed greater anti-inflamma-tory effects,dose-dependently inhibiting the development of ankle swelling in rats(P<0.01,P<0.001)and inhibited inflammatory cy-tokine infiltration.RA inhibited the secretion of inflammatory cytokines IL-6,IL-1β,TNF-α(P<0.01)and inhibited the expressions of proteins associated with the NF-κB/NLRP3 pathway(P<0.01).Conclusion:RA shows potent anti-inflammatory effects in a mouse model of AGA,and the mechanism is associated with regulation of the NF-κB/NLRP3 pathway.
