Research progress of genomic DNA in spent embryo culture media and the blastocoel fluid in preimplantation genetic testing
10.3760/cma.j.cn101441-20200707-00381
- VernacularTitle:胚胎培养液和囊胚腔液中基因组DNA在胚胎植入前遗传学检测中的研究进展
- Author:
Xiaodan WANG
1
;
Suibing MIAO
1
;
Xiaohua WU
1
Author Information
1. 石家庄市第四医院（河北医科大学附属妇产医院），石家庄 050000
- Publication Type:Journal Article
- Keywords:
Cell-free nucleic acids;
Preimplantation genetic testing;
Spent embryo culture media;
Blastocoel fluid
- From:
Chinese Journal of Reproduction and Contraception
2022;42(3):311-317
- CountryChina
- Language:Chinese
-
Abstract:
Chromosomal abnormality of embryos is one of the main causes of pregnancy failure. Preimplantation genetic testing (PGT) can significantly improve the implantation rate and the rate of continuous pregnancy by selecting chromosomally normal embryos to transfer, but the invasive nature and operational complexity of PGT limit its clinical application. Therefore, the development of a non-invasive, rapid and economical method for embryo genome detection would represent a progress in assisted reproduction. In recent years, with the discovery of cell-free genomic DNA in spent embryo culture media and the blastocoel fluid, genetic analysis of cell-free DNA has been reported in evaluating the genetic constitution of embryos, however, the published studies lack consistent conclusions. In this review, we have summarized the progress of cell-free genomic DNA in PGT and discussed its current limitations and future clinical implications.
