Association between morphological parameters and ploidy status of the blastocysts
10.3760/cma.j.cn101441-20200421-00234
- VernacularTitle:胚胎形态学参数与整倍体状态的相关性分析
- Author:
Chunyan SHEN
1
;
Jianrui ZHANG
1
;
Shanshan DU
1
;
Yanli LIU
1
;
Yichun GUAN
1
;
Rujing YANG
1
;
Xingling WANG
1
Author Information
1. 郑州大学第三附属医院生殖医学科 450052
- Publication Type:Journal Article
- Keywords:
Blastocysts;
Euploid;
Preimplantation genetic testing;
Biopsy time
- From:
Chinese Journal of Reproduction and Contraception
2021;41(6):522-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between the human Day 3 embryo quality, blastocyst biopsy time, degree of expansion of the blastocysts, inner cell mass (ICM) morphology, trophectoderm (TE) morphology and their ploidy status.Methods:This was a retrospective case-control study for patients who underwent in vitro fertilization with preimplantation genetic testing (PGT) at the Reproductive Medicine Center of the Third Affiliated Hospital of Zhengzhou University from January 2017 to October 2019. A total of 977 blastocysts were biopsied in 255 cycles of PGT, and the correlation between relevant morphological parameters and euploidy rate was analyzed. Results:1) A total of 977 blastocysts from 255 cycles were biopsied, and 937 blastocysts (95.9%) successfully obtained chromosome results, including 396 (42.3%) euploidy and 541 (57.7%) aneuploidy. 2) There was no significant correlation between the euploidy rate of blastocysts and the maternal age in the preimplantation genetic testing for chromosomal structural rearrangements (PGT-SR) cycles (633 blastocysts), while in the preimplantation genetic testing for aneuploidy (PGT-A) cycles (304 blastocysts) the euploidy rate of blastocysts showed a significant downward trend with the increase of the maternal age ( P=0.000 1). 3) The euploidy rate of blastocysts biopsy on day 6 and day 7 was significantly lower than that on the day 5 ( OR=0.7, 95% CI=0.5-0.9, P=0.009 1; OR=0.4, 95% CI=0.1-0.6, P=0.001 2), the euploidy rate of the blastocysts with ICM grade B was significantly lower than that of the blastocysts with ICM grade A ( OR=0.4, 95% CI=0.3-0.7, P=0.000 1), the rate of euploidy of the blastocysts with TE grade C was significantly lower than that with TE grade A ( OR=0.4, 95% CI=0.2-0.7, P=0.000 4) adjusting for maternal age and cycle difference, while day 3 embryo quality, degree of expansion of the blastocysts were not associated with blastocysts ploidy. Conclusion:Day 3 embryo quality and expansion of the blastocysts have no correlation with euploidy, while the blastocyst biopsy time, ICM morphology, TE morphology are significantly correlated with their ploidy status.
