Study on the Mechanism of Xieke Decoction in Treating Polycystic Ovary Syndrome Based on the"Ke-Nang Theory"
10.14148/j.issn.1672-0482.2025.1011
- VernacularTitle:基于"窠囊理论"探讨泻窠汤治疗多囊卵巢综合征的机制研究
- Author:
Yinxing ZHU
1
;
Yan CHEN
;
Dandan ZHU
;
Zheng YU
Author Information
1. 南京中医药大学泰州附属医院,江苏 泰州 225300
- Publication Type:Journal Article
- Keywords:
Ke-Nang theory;
Xie Ke Decoction;
polycystic ovary syndrome;
network pharmacology;
molecular docking;
clinical research;
intermingled phlegm and blood stasis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(8):1011-1020
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the potential mechanisms of Xie-Ke Decoction,based on the"Ke-Nang theory",in treating polycystic ovary syndrome(PCOS)and to identify its potential therapeutic targets,thereby providing a theoretical basis for the applica-tion of the"Ke-Nang theory"in PCOS treatment.METHODS The components of Xie-Ke Decoction and its potential targets for P-COS treatment were retrieved from databases such as TCMSP,SwissTargetPrediction,GeneCards,CTD,and GEO.A component-tar-get network was constructed using Cytoscape,and GO and KEGG enrichment analyses were performed to elucidate its mechanisms of action.LASSO and Wilcoxon tests were used to screen and validate key targets,and molecular docking was conducted to verify the binding affinity between core components and key targets.Clinical samples were collected,and ELISA was used to validate the predic-ted targets.RESULTS A total of 29 core genes were identified,with GO enrichment analysis involving pathways such as inflammato-ry response,metabolic regulation,and extracellular matrix remodeling.KEGG enrichment analysis covered pathways like cancer path-ways,inflammatory pathways,insulin resistance,and lipid metabolism pathways.Five effective targets were ultimately screened and validated:CTSL,FABP5,HMOX1,PIK3CD,and MMP9.The core component quercetin showed strong affinity for MMP9.Clinical studies revealed that the number of retrieved oocytes and the levels of MMP-9 in follicular fluid were significantly higher in the Xie-Ke Decoction treatment group than in the control group(P<0.05).CONCLUSION The therapeutic targets and pathways of Xie-Ke De-coction in treating PCOS are revealed by network pharmacology and verified by molecular docking and clinical research,providing sci-entific evidence for its mechanism of action.It also lays a theoretical foundation for the clinical application and pharmacological re-search of the"Ke-Nang theory".
