Analysis of DNA methylation status of DAZL in semen from men with oligoasthenozoospermia
10.3760/cma.j.cn101441-20200330-00171
- VernacularTitle:少弱精子症患者 DAZL基因DNA甲基化分析
- Author:
Peng XUE
1
;
Kunpeng WANG
;
Jinbao GU
;
Daoyuan LU
;
Hui WANG
;
Xiaobao ZHANG
Author Information
1. 徐州医科大学附属连云港医院泌尿外科 222000
- Publication Type:Journal Article
- Keywords:
Male infertility;
DAZL gene;
Oligoasthenozoospermia;
DNA methylation;
Bisulfite genomic sequencing PCR
- From:
Chinese Journal of Reproduction and Contraception
2021;41(4):327-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the DNA methylation levels of DAZL promoter regions in spermatozoa between the normozoospermic and oligoasthenozoospermic males. Methods:A case-control study was performed. Routine semen analysis and morphological analysis were performed in 15 normozoospermic individuals (control group) and 35 subfertile patients with oligoasthenozoospermia(oligoasthenozoospermia group). Semen were purified by density gradient centrifugation. DNA was extracted, treated with bisulfite, amplified by PCR and the purified PCR products were cloned into pCR2.1 vector and then proceeded to chemical transformation, restriction enzyme reaction. The positive clones of each sample were selected for sequencing analysis and DNA methylation analysis.Results:The DNA methylation rate of DAZL promoters was increased significantly in oligoasthenozoospermia group (12.15%±11.35%) compared with control group (0.96%±0.46%) ( P=0.000 4). There was also an obvious difference in the DNA methylation status among oligoasthenozoospermia males. DNA methylation levels were significantly increased in 12 (34.3%) patients with oligoasthenozoospermia. Conclusion:Aberrant methylation of DAZL gene promoters is associated with male infertility, and it may be a biomarker of human spermatogenesis defect.
