Effect of GS-9620 in imiquimod-induced psoriasis-like inflammation in mice based on gut microbiota
10.3969/j.issn.1005-4847.2025.07.009
- VernacularTitle:基于肠道菌群探讨GS-9620改善IMQ诱导的小鼠银屑病样炎症的研究
- Author:
Jingyu YANG
1
;
Qian ZHANG
;
Si CHEN
;
Xiaotang WANG
;
Guohua SONG
Author Information
1. 山西医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室,太原 030001
- Publication Type:Journal Article
- Keywords:
psoriasis;
psoriasis-like inflammation animal models;
gut microbiota;
GS-9620;
16S rRNA
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(7):1021-1031
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of GS-9620 in improving imiquimod(IMQ)-induced psoriasis-like inflammation by regulating the Th1/Th17-related immune response,and to investigate its regulatory effect on the gut microbiota in mice.Methods An IMQ-induced psoriasis-like inflammation model was established in BALB/c mice.The severity of the skin lesions was evaluated by psoriasis area and severity index(PASI)score.The proportions of CD4+interleukin(IL)-17+and CD4+interferon(IFN)-γ+cells in spleen tissue were detected by flow cytometry.Levels of the inflammatory factors tumor necrosis factor(TNF)-α,IL-1β,and IL-6 in skin tissues were determined by enzyme-linked immunosorbent assay,and pathological analysis was performed by hematoxylin/eosin staining.The effects of GS-9620 on the structure of the gut microbiota in control,IMQ model,and GS-9620-treated mice were detected by 16S rRNA sequencing.Results GS-9620 significantly reduced the PASI score in IMQ-induced mice and effectively reduced the proportions of CD4+IL-17+and CD4+IFN-γ+cells in the spleen.GS-9620 also significantly down-regulated the expression levels of TNF-α,IL-1β,and IL-6 in skin tissues.16S rRNA sequencing showed that GS-9620 significantly regulated the abundance of gut microbiota related to inflammation,including the relative abundances of bacteria such as Lachnospiraceae_NK4A136_group,Lachnospiraceae_UCG-008,Alloprevotella,Desulfovibrio,Prevotellaceae_UCG-001,and Alistipes.Conclusions GS-9620 effectively alleviates IMQ-induced psoriasis-like skin inflammation in mice by regulating the expression of Th1/Th17-related inflammatory factors.It may also improve IMQ-induced clinical symptoms by regulating the structure of the gut microbiota,thus providing a new theoretical basis for the treatment of psoriasis.The result of this study provide important experimental evidence to support further investigations into the application of GS-9620 for the treatment of psoriasis.
