Research progress in differentiation of male germ cells derived from induced pluripotent stem cells in vitro
10.3760/cma.j.cn101441-20200111-00021
- VernacularTitle:诱导多能干细胞体外分化为雄性生殖细胞的研究进展
- Author:
Shanshan WU
1
;
Wenjuan FAN
;
Ruiling LI
;
Jinbo DENG
Author Information
1. 河南大学护理与健康学院，开封 475000
- Publication Type:Journal Article
- Keywords:
Pluripotent stem cells;
Male germ cells;
Spermatogenesis;
Differentiation in vitro
- From:
Chinese Journal of Reproduction and Contraception
2021;41(1):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Male infertility is an important issue affecting reproductive health, and its etiology and mechanism are complex or not clear. For patients with azoospermia, specific clinical treatments are limited. Therefore, to generate patients' own functional sperms is absolutely necessary. With the development of techniques, the scientists start to think about how to attain functional sperms deriving from human induced pluripotent stem cells (iPSCs) in vitro. Mainly, sperm induction with iPSCs includes two steps, first, the formation of diploid primordial germ cell, then the differentiation of haploid mature sperms. Technically, the difficulties lie in the generation of meiosis from a diploid cell into haploid sperm, and unfortunately, the problem is not solved yet. In order to understand this kind of knowledge well, we try to review the technical progress and principle of male gamete induction from iPSCs. Hopefully, it can help us to understand the mechanism of spermatogenesis in vitro well, and to provide a reference for the clinical application of in vitro fertilization.
