Effects of Ang1-7, A779 and Ang Ⅱ on insulin signaling pathway proteins in ovarian granulosa cells of insulin resistant rat
10.3760/cma.j.cn101441-20200106-00007
- VernacularTitle:Ang1-7、A779、AngⅡ对胰岛素抵抗的大鼠卵巢颗粒细胞胰岛素信号通路蛋白的影响
- Author:
Si CHEN
1
;
Xiuli WANG
;
Xiaojing HE
;
Yuan ZHANG
;
Yiqian WANG
;
Xiaodong LI
Author Information
1. 河北医科大学第二医院妇科，石家庄 050000
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Insulin resistance;
Angiotensin Ⅱ
- From:
Chinese Journal of Reproduction and Contraception
2021;41(2):131-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the changes of glucose and insulin signaling pathway proteins in rat ovarian insulin resistant granulosa cells by detecting Ang1-7, MAS receptor inhibitors A779 and Ang Ⅱ.Methods:First, rat ovarian granulosa cells were cultured with dexamethasone and insulin to establish the insulin resistance (IR) model, and the concentration differences of glucose and lactic acid in both blank and IR model groups were detected. According to the different drugs treated, the cells in the IR model group were divided into Ang Ⅱ group, Ang1-7 group, Ang1-7+Ang Ⅱ group, A779 group, Ang1-7+A779 group. After 24 h, the concentration differences of glucose of all groups were tested. At last, Western blotting was used to detect the expressions of P-Akt/Akt, P-Gsk-3β/GSK-3β, P-AS160/AS160 in the Mas receptor protein and key proteins in the insulin signaling pathway in the above groups.Results:1) Compared with the blank group, the concentration differences of glucose and lactic acid of the supernatant were reduced after applying dexamethasone and insulin to rat ovarian granulosa cells, suggesting that the IR model was successfully established. 2) Compared with the model group, the concentration difference of glucose in the Ang1-7 group was increased (5.55±0.21, P=0.002 1), while the Ang Ⅱ subgroup was decreased. There was no significant difference of the concentration difference of glucose between the Ang1-7+Ang Ⅱ group and the A779 group ( P>0.05). However, the concentration difference of glucose in the Ang1-7+A779 group was decreased (4.89±0.20, P=0.010 8). 3) There was no difference in the expressions of Akt, GSK-3β, AS160 among the black group,model group and the different drugs treated groups. Compared with the model group, the expressions of P-Akt, P-GSK-3β, and P-AS160 in the Ang1-7 group were enhanced, while the expressions of P-Akt, P-GSK-3β, and P-AS160 in the Ang Ⅱ group were decreased. And compared with Ang1-7 group, Mas receptor expression in Ang1-7+A779 group was decreased. Conclusion:1) The effect of Ang1-7 on improving the IR condition of rat ovarian granulosa cells in IR model was opposite to Ang Ⅱ, which was achieved by improving the expression of the phosphorylation expression of Akt, GSK-3β, AS160 proteins in insulin signaling pathway. 2) The addition of A779 can inhibit the improvement of Ang1-7 on Mas, glucose metabolism and insulin signaling pathway, which meant in the role of Ang1-7 on improvement of the glucose metabolism, its downstream Mas receptor was involved in the process.
