Establishment of Content Determination Method of Zhihuoding Granules Based on Anti-inflammatory Activity and Fingerprint
10.3870/j.issn.1004-0781.2025.07.002
- VernacularTitle:基于抗炎活性与指纹图谱建立栀藿定颗粒含量测定方法
- Author:
Xin ZENG
1
;
Guangzhi SHAN
;
Wenling SU
;
YIDERESI·MOLA
;
Yue WANG
;
Jianguo XING
Author Information
1. 石河子大学药学院,石河子 832003;新疆维吾尔自治区药物研究所,乌鲁木齐 830011
- Publication Type:Journal Article
- Keywords:
Zhihuoding granules;
Tumor necrosis factor-α;
High performance liquid chromatography;
Fingerprint chromatogram;
Content determination
- From:
Herald of Medicine
2025;44(7):1028-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the content determination method of Zhihuoding granules(ZHDG)by the anti-inflammatory active ingredients and fingerprint.Methods The inflammatory model of BEAS-2B cells was established by LPS(10 μg·mL-1)stimulation for 24 h,and the level of TNF-α in the supernatant was detected by enzyme-linked immunosorbent assay(ELISA).Using the reverse high-performance liquid chromatography(RP-HPLC)of 7 batch ZHDG fingerprint;HPLC was used to determine the content of geniposide and baicalin in granules.Results Compared with LPS Group,ZHDG,prim-O-glucosylcimifugin,5-O-methylvisammioside,baicalin,geniposide,and total polysaccharide extract all significantly decreased TNF-αlevels in BEAS-2B cells induced by LPS(P＜0.05).Fingerprint spectra of 7 batches of ZHDG were established,with a similarity of 1.000 to the reference.A total of 15 peaks were calibrated,and 7 chromatographic peaks were identified.Gardenin and baicalin were selected as the index components of ZHDG for evaluation.The established method was rapid and accurate.Conclusion The method for the determination of baicalin and geniposide in ZHDG are exclusive,accurate,and effective,providing reference for the quality control and clinical application of ZHDG.
