Effect of Ziyin Bushen sequential method on acute regulatory proteins of steroid hormone and SIRT1 gene expression in ovaries of polycystic ovary syndrome rats
10.3760/cma.j.cn101441-20191202-00537
- VernacularTitle:滋阴补肾序贯法对多囊卵巢综合征大鼠卵巢StAR蛋白及 SIRT1基因表达的影响
- Author:
Wei LIN
1
;
Hongyang ZHU
;
Yangyang MEI
;
Changlong FU
;
Xiaomin HUANG
;
Yan YE
;
Lei DONG
Author Information
1. 福建卫生职业技术学院，福州 350101
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Ziyin Bushen;
Sequential Method;
Steroids acute regulatory synthesis protein;
Silent information regulator 1
- From:
Chinese Journal of Reproduction and Contraception
2020;40(11):914-920
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ziyin Bushen sequential method on the expression of steroids acute regulatory synthesis protein (StAR) and silent information regulator 1 (SIRT1) in ovaries of polycystic ovary syndrome (PCOS) rats.Methods:PCOS rat model was established by dehydroepiandrosterone (DHEA). Eighty 21-day-old clean SD rats were randomly divided into control group (16 rats) and PCOS group (64 rats). PCOS group was further divided into normal saline (NS) group, Ziyin group, Bushen group and Xuguan group with 16 rats in each group. Each group was given intragastric administration for 20 d. Serum estradiol, progesterone and testosterone levels were measured by enzyme linked immunosorbent assay (ELISA) method. The expression levels of StAR, SIRT1 protein and mRNA were detected by Western blotting and RT-PCR.Results:The serum estradiol level in normal saline group was significantly higher than that in control group ( P=0.004). The levels of serum estradiol in Ziyin group, Bushen group and Xuguan group were significantly lower than that in NS group ( P=0.005, P=0.003 and P=0.002, respectively). The serum testosterone level in normal saline group was significantly higher than that in control group ( P=0.005); the level of serum testosterone in Xuguan group was significantly lower than that in NS group, Ziyin group and Bushen group ( P=0.002, P=0.005 and P=0.005, respectively). Compared with control group, the StAR protein content in the normal saline group was higher ( P<0.001), while SIRT1 mRNA was significantly decreased ( P<0.001). Compared with NS group, Ziyin group and Bushen group, the expression of StAR protein and mRNA in Xuguan group was significantly lower ( P<0.001, P=0.008 and P<0.001, respectively); the expression of SIRT1 protein in Xuguan group was significantly higher than that in NS group and Bushen group (all P<0.001); compared with the control group, StAR mRNA in NS group was significantly increased ( P<0.001), while SIRT1 mRNA was significantly decreased ( P<0.001); the expression of SIRT1 gene in Ziyin group and Xuguan group was significantly higher than that in NS group (all P<0.001), while that in Xuguan group was significantly higher than that in Bushen group ( P<0.001). Conclusion:Ziyin Bushen sequential method may decrease the serum androgen level of PCOS rats by upregulating SIRT1 gene expression and StAR in ovary expression.
