Impacts of Yiqi Wenfei Tongqiao Formula on inflammatory response and Th1/Th2 immune balance in rats with lung Qi deficiency cold type allergic rhinitis
10.3969/j.issn.1000-484X.2025.09.027
- VernacularTitle:益气温肺通窍方对肺气虚寒型变应性鼻炎大鼠炎症反应及Th1/Th2免疫平衡的影响
- Author:
Shan ZHU
1
;
Wenjin ZHAO
1
;
Xinlei TIAN
1
;
Yijin ZHOU
1
;
Zhipeng ZHU
1
;
Wenjie HU
1
Author Information
1. 河南省中医院暨河南中医药大学第二附属医院儿科,郑州 450002
- Publication Type:Journal Article
- Keywords:
Yiqi Wenfei Tongqiao Formula;
Lung Qi deficiency cold type allergic rhinitis.;
Inflammation;
Th1/Th2;
Immune balance
- From:
Chinese Journal of Immunology
2025;41(9):2220-2225
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the impacts of Yiqi Wenfei Tongqiao Formula on inflammatory response and T helper cell(Th)1/Th2 immune balance in rats with lung Qi deficiency cold type allergic rhinitis.Methods:Sixty SD rats were randomly divided into model group,low dose Yiqi Wenfei Tongqiao Formula group,medium dose Yiqi Wenfei Tongqiao Formula group,high dose Yiqi Wenfei Tongqiao Formula group,and desloratadine group,with 12 rats in each group,using a combination of smoking,cold stimula-tion combined with ovalbumin(OVA)sensitization and stimulation construct lung Qi deficiency cold type allergic rhinitis model.An-other 12 normal SD rats were selected as control group.Each group of rats was treated with corresponding drugs once a day for 14 days.Model group and control group rats were given physiological saline.Scoring method was used to analyze the behavioral score and lung index of rats with allergic rhinitis.HE staining was applied to detect pathological changes in rat nasal mucosa tissue.ELISA was ap-plied to detect levels of IFN-γ,IL-4 and TNF-α in rats serum;flow cytometry was applied to detect Th1/Th2.Western blot was applied to detect expressions of T-bet and GATA-3 protein in nasal mucosa tissues of rats in each group.Results:Compared with control group,pathological damage to nasal mucosa tissue of model group rats was severe,allergic rhinitis behavior score(days 41,48,56),lung index,levels of IL-4 and TNF-α,Th2 and protein expression of GATA-3 were increased,while levels of IFN-γ,Th1,Th1/Th2,and protein expression of T-bet were decreased(P<0.05);compared with model group,pathological damage to nasal mucosa tissue of rats in low,medium,high doses Yiqi Wenfei Tongqiao Formula groups and desloratadine group were alleviated,the allergic rhinitis behavior score(days 48,56),lung index,levels of IL-4 and TNF-α,Th2 and protein expression of GATA-3 were decreased,while levels of IFN-γ,Th1,Th1/Th2,and protein expression of T-bet were increased(P<0.05).Conclusion:Yiqi Wenfei Tongqiao Formula can inhibit inflammatory response of lung Qi deficiency cold type allergic rhinitis rats By regulating Th1/Th2 immune balance.
