Mesothelin-specific T-cell immune reconstitution after hematopoietic stem cell transplantation in acute myeloid leukemia
10.3969/j.issn.1000-484X.2025.09.030
- VernacularTitle:急性髓系白血病造血干细胞移植后间皮素特异性T细胞免疫重建初步研究
- Author:
Xiaohua LUO
1
;
Yuting CHEN
1
;
Lan WANG
1
;
Wen PENG
1
;
Xiaoqin DUAN
1
;
Liping SHUI
1
Author Information
1. 重庆医科大学附属第一医院血液科,重庆 400016
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Acute myeloid leukemia;
Mesothelin;
T lymphocyte;
Immune reconstitution
- From:
Chinese Journal of Immunology
2025;41(9):2237-2242
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the pattern of Mesothelin(MSLN)-specific T-cell(CTLs)immune reconstitution after allogeneic hematopoietic stem cell transplantation.Methods:Two cases of Mesothelin-positive patients with acute myeloid leukemia(AML)were screened,and the number of MSLN-CTLs and its subpopulations,the expression of surface PD-1/CTLA-4/TIM-3 im-mune exhaustion molecules,and the functions of secreted cytokines TNF-α and IFN-γ were monitored by flow cytometry after trans-plantation.They were compared with WT1-CTLs and cytomegalovirus(CMV)CTLs.Meanwhile,the relationship between CTL recon-stitution and Minimal residual disease(MRD)and leukemia recurrence was analyzed.Results:After transplantation,MSLN-CTLs,WT1-CTLs and CMV-CTLs can be detected,and intracellular factors were secreted.The phenotypes of WT1 specific CD8+T cells,MSLN specific CD8+T cells and CMV specific CD8+T cells were mainly TEM subsets and TEMRA subsets,and the TEM subsets of CMV specific CD8+T cells were more obvious.Compared with CMV-CTLs,the proportions of T Naive,TCM and TEMRA subsets were relatively higher in MSLN-specific CD8+T cells and WT1-specific CD8+T cells,and the expression levels of PD-1,CTLA-4 and TIM-3 were higher in MSLN-CTLs and WT1-CTLs.The dynamic changes of MSLN-CTLs and WT1-CTLs after transplantation were related to leukemia load and the chimerism rate of donor and recipient.Conclusion:After allogeneic hematopoietic stem cell transplantation,im-mune recovery to MSLN is found,which is different from WT1-CTLs and CMV-CTLs.
