Two-sample Mendelian randomization of causal relationship between intestinal flora and ankylosing spondylitis
10.3969/j.issn.1000-484X.2025.09.002
- VernacularTitle:肠道菌群和强直性脊柱炎因果关系的两样本孟德尔随机化研究
- Author:
Renjie LU
1
;
Wenjun SHI
1
;
Shaoyang LIU
1
;
Shuo GE
1
;
Guangyue LIU
1
;
Jixiang SHI
1
Author Information
1. 上海中医药大学附属普陀医院,上海 200062
- Publication Type:Journal Article
- Keywords:
Ankylosing spondylitis;
Intestinal flora;
Mendelian randomization analysis;
Causality;
Genetic variation
- From:
Chinese Journal of Immunology
2025;41(9):2055-2060
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate causal relationship between intestinal flora and risk of developing ankylosing spondylitis(AS)by a two-sample Mendelian randomization(MR)analysis.Methods:Genome-wide association study(GWAS)data on 211 types of intestinal flora and AS were obtained,and single nucleotide polymorphism(SNP)was used as an instrumental variable and sensi-tive SNPs were selected for analysis.Two-sample MR analyses were performed by inverse variance weighted(IVW)as well as MR-Eg-ger,Weighted median,to assess causal relationship between AS and intestinal flora by OR,and results were tested for heterogeneity and pleiotropy.Results:IVW analysis showed a significant causal relationship between 11 intestinal flora and AS risk.Among them,Verrucomicrobiae,Verrucomicrobiales,Verrucomicrobiaceae,Akkermansia,Erysipelatoclostridium,Holdermannia,Holdemania,Bacillales and Verrucomicrobia had positive causal effect on AS risk,while Dialister,Howardella and Oscillospira had negative causal effect.Causal effect estimates obtained by different methods(MR-Egger,Weighted median)were consistent,and sensitivity tests did not reveal significant horizontal pleiotropy and heterogeneity.Conclusion:Intestinal flora plays an important role in pathogenesis of AS,while specific mechanism remains to be further investigated.
