Study of neuroprotective effect of nicotinamide riboside on EAE mice
10.3969/j.issn.1000-484X.2025.09.001
- VernacularTitle:烟酰胺核糖对实验性自身免疫性脑脊髓炎小鼠的神经保护作用研究
- Author:
Guoping XI
1
;
Guobin SONG
;
Yanhua LI
;
Tao MENG
;
Jiwei WANG
;
Qin SU
;
Siwei JIA
;
Yi GUO
;
Qing WANG
;
Cungen MA
Author Information
1. 山西大同大学脑科学研究所,大同 037009
- Publication Type:Journal Article
- Keywords:
Nicotinamide riboside;
Experimental autoimmune encephalomyelitis;
Neuroprotection;
Neurotrophic factor
- From:
Chinese Journal of Immunology
2025;41(9):2049-2054
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neuroprotective effect of nicotinamide riboside(NR)on experimental autoimmune en-cephalomyelitis(EAE)mice.Methods:C57BL/6 female mice were induced by myelin oligodendrocyte glycoprotein(MOG35-55)to pro-duce EAE model and were randomly divided into EAE group and NR group.From day 3 to day 27 after immunization,each mouse in EAE group was given normal saline(200 μl/d)and each mouse in NR group was given NR(500 mg/kg,200 μl/d)by intragastric administration.Clinical score and body weight of mice in EAE group and NR group were recorded every day.On the 28th day after immunization,the spinal cord protein of mice in each group was extracted and the frozen sections of spinal cord of mice in each group were prepared.LFB staining was used to detect demyelination,immunofluorescence staining was used to detect the expression of MAP-2 and the number of positive cells of NeuN,BDNF,GDNF,NGF and NT-3,and Western blot was used to detect the expressions of BDNF,GDNF,NGF and NT-3 of spinal cord.Results:Compared with EAE group,NR significantly delayed the onset time of EAE mice(P<0.05),decreased clinical score(P<0.05),reduced weight loss,alleviated spinal cord demyelination(P<0.05),increased the expression of MAP-2(P<0.01)and the number of NeuN positive cells(P<0.01),and up-regulated the expressions of BDNF,GDNF,NGF and NT-3(P<0.05).Conclusion:NR shows a good neuroprotective effect on EAE mice.The mechanism may be related to NR significantly increasing the expression of spinal neurotrophic factors,improving the microenvironment of the central nervous sys-tem,nourishing nerves,promoting nerve repair and nerve growth,etc.
