Application of preimplantation genetic testing in treatment of infertile couples with abnormal chromosome structure
10.3760/cma.j.cn101441-20190529-00215
- VernacularTitle:胚胎植入前遗传学检测在染色体结构异常不孕夫妇治疗中的应用
- Author:
Min PANG
1
;
Jingwen LIU
1
;
Qiongzhen ZHAO
1
;
Kun ZHANG
1
;
Weijuan LI
1
;
Weidong HUANG
1
Author Information
1. 新疆佳音医院生殖医学中心，乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
Preimplantation genetic testing;
Chromosome structure abnormality;
Next generation sequencing
- From:
Chinese Journal of Reproduction and Contraception
2020;40(7):573-578
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the application of preimplantation genetic testing (PGT) in the treatment of infertile couples with abnormal chromosome structure, and provide a reference for the promotion and application of PGT technology.Methods:The clinical data of the first batch of patients who received PGT due to chromosomal structural abnormalities in Xinjiang JIAYIN Reproductive Medicine Center from June 2017 to July 2019 were retrospectively analyzed. According to the abnormal type, the patients were divided into three groups: mutual translocation, Roche translocation and inversion. After intracytoplasmic sperm injection (ICSI), the available blastocysts were selected for external trophoblast cell biopsy and next generation sequencing analysis of chromosome of embryonic cells. Embryos with normal detection results were selected for single blastocyst thawing and transplantation. The patient's general information, ovulation induction and embryo culture, blastocyst biopsy results and pregnancy outcome after transplantation were compared among the three groups. Results:A total of 51 patients completed 67 PGT cycles, a total of 189 blastocysts were genetically detected. There were significant differences among the three groups in the ratio of complex abnormal embryos and aneuploidy embryos (all P<0.001), but no significant differences were observed in other data ( P>0.05). There were 16 successful pregnancies out of 33 successful transplant cycles, with a pregnancy rate of 48.5%, and 7 patients had successfully given birth to healthy babies. Conclusion:The clinical application of PGT can effectively improve the fertility of couples with abnormal chromosome structure, and the proportion of embryo abnormalities of different types of carriers is different, which can be used as a genetic reference.
