Expression of immune function-related molecules in CD4+CD28-T cell subset in peripheral blood of rheumatoid arthritis patients
10.3969/j.issn.1000-484X.2025.09.007
- VernacularTitle:类风湿关节炎患者外周血CD4+CD28-T细胞亚群免疫功能相关分子的表达分析
- Author:
Qian LIN
1
;
Cheng ZHANG
1
;
Jie ZHANG
1
;
Huina HUANG
1
;
Xia LI
1
;
Guan WANG
1
Author Information
1. 大连医科大学基础医学院免疫学教研室,大连 116044
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
CD4+CD28-T cells;
Toll-like receptor 2;
TNF-α;
Perforin
- From:
Chinese Journal of Immunology
2025;41(9):2087-2091
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the activation pathway of CD4+CD28-T cells and their specific role in rheumatoid arthritis(RA).Methods:Peripheral blood mononuclear cells(PBMCs)were isolated from RA patients and healthy individuals.Flow cytome-try was used to detect the proportion and activation level of CD4+CD28-T cells,as well as the expressions of perforin and TNF-α,and to analyze the correlation between the proportion of CD4+CD28-T cells and age,C-reactive protein,anti-cyclic citrullinated peptide(CCP)antibodies,rheumatoid factors(RF)and erythrocyte sedimentation rate(ESR).The expression of Toll-like receptor(TLR)2 on CD4+CD28-T cells was detected by flow cytometry.Purified RA CD4+T cells were stimulated with anti-CD3 antibody and TLR2 agonist alone or in combination,and the activation level and cytokine secretion of CD4+CD28-T cells were detected by flow cytometry.Results:The proportion of CD4+CD28-T cells in RA patients was higher than that in healthy individuals(P<0.05),and was positively correlated with rheumatoid factors and erythrocyte sedimentation rate(r=0.554 1,P<0.01;r=0.363 4,P<0.01).Compared with CD4+CD28+T cells,CD25 expression was downregulate(P<0.01),but CD40L and TLR2 expressions were upregulated(P<0.01;P<0.000 1)in CD4+CD28-T cells from RA patients,and they secreted more perforin and TNF-α(P<0.001;P<0.001).After TLR2 ago-nist stimulation,there was no significant change in CD40L and CD25 expression,as well as perforin and TNF-α secretion in CD4+CD28-T cells from RA patients.Conclusion:The proportion of CD4+CD28-T cells is increased in RA patients,and they secrete a large amount of perforin and TNF-α,which may be involved in the inflammatory state and joint damage of RA,but TLR2 cannot re-place CD28 in activating CD4+CD28-T cells.
