The effect of sevoflurane on neuronal inflammation in rats with spinal cord injury by regulating the ROS/TXNIP/NLRP3 pathway
- VernacularTitle:七氟醚调节ROS/TXNIP/NLRP3通路对脊髓损伤大鼠神经细胞炎症的影响
- Author:
Yuke ZHOU
1
;
Wei SUN
1
;
Qing GAO
1
;
Jun HE
1
Author Information
- Publication Type:Journal Article
- Keywords: sevoflurane; spinal cord injuries; reactive oxygen species; thioredoxins; NLR family,pyrin domain-containing 3 protein; neurons; inflammation
- From: Tianjin Medical Journal 2025;53(9):910-915
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of sevoflurane on neuroinflammation in rats with spinal cord injury by regulating the reactive oxygen species(ROS)/thioredoxin-interacting protein(TXNIP)/nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)pathway.Methods Seventy-two rats were randomly divided into six groups using a random number table method,with 12 rats in each group:the model group(establishing the rat model of spinal cord injury),the sham operation group,the low-,medium-and high-concentration sevoflurane groups(1%,2%and 4%sevoflurane,respectively)and the combination group(4%sevoflurane+3.7 mg/kg reactive oxygen species(ROS)activator trimethylamine N-oxide).Spinal cord injury behavior(BBB)score and footprint imprint test were used to evaluate motor function.ELISA method was used to detect the levels of IL-18,TNF-α and IL-1β in spinal cord tissue.HE staining was used to detect pathological changes in spinal cord tissue.TUNEL method was used to detect cell apoptosis.In addition,the expression of TXNIP and NLRP3 mRNA were detected by qRT-PCR.ROS activity was detected by ROS kit.Western blot assay was used to detect the expression of ROS/TXNIP/NLRP3 pathway proteins.Results The sham operation group showed normal gait,normal cell morphology with orderly arrangement and intact spinal cord structure.The model group presented with toe dragging,severe spinal cord tissue injury and inflammatory cell infiltration.The BBB score was lower in the model group compared to that of the sham surgery group,while IL-18,TNF-α,IL-1β levels,apoptosis rate,ROS activity,TXNIP,NLRP3 mRNA and protein expression were increased(P＜0.05).The above indexes were improved in the low-concentration,medium-concentration and high-concentration sevoflurane groups compared with those in the model group.However,the combined group reversed the improvement results of the high-concentration sevoflurane group.Conclusion Sevoflurane can alleviate neuroinflammation in rats with spinal cord injury by inhibiting the ROS/TXNIP/NLRP3 pathway.
