Correlation between changes of mTOR signaling pathway in granulosa cells and reproductive endocrine abnormalities in polycystic ovary syndrome patients
10.3760/cma.j.cn101441-20190326-00122
- VernacularTitle:多囊卵巢综合征患者颗粒细胞mTOR通路改变与生殖内分泌异常的相关性
- Author:
Yingxuan MA
1
;
Haiyun GUAN
1
;
Lu WANG
1
;
Wei ZHANG
1
Author Information
1. 复旦大学附属妇产科医院，上海 200011
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
mTOR signaling;
Testosterone;
Granulosa cells
- From:
Chinese Journal of Reproduction and Contraception
2020;40(4):287-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the changes of mTOR signaling molecules in granulosa cells from polycystic ovary syndrome (PCOS) patients, and whether the change of mTOR siganaling is related to serum hormones.Methods:The phosphorylation levels of mTOR signaling molecules in primary granulosa cells were compared between PCOS group and control group. The correlation between phosphorylation levels of mTOR signaling and serum hormones was also analyzed. Then effects of exogenous testosterone and insulin on mTOR signaling were investigated in vitro. Results:Granulosa cells from PCOS patients (0.66±0.29) had higher phosphorylation levels of mTOR compared with control group (0.34±0.32, P=0.02). The level of p-s6k1 in PCOS group (0.95±0.42) was higher than that in control group (0.62±0.29), but the difference was not statistically significant ( P>0.05). The serum testosterone was associated with p-mTOR and p-S6k1 ( P=0.01, P=0.01). Serum follicle-stimulating hormone (FSH) ( P=0.67, P=0.75) and luteinizing hormone (LH) ( P=0.75, P=0.37) levels were irrelevant with p-mTOR and p-S6k1. Conclusion:Granulosa cells from PCOS patients had higher activated mTOR signaling. Testosterone and insulin could elevate the phosphorylation levels of mTOR signaling of granulosa cells in vitro, which maybe contributed to the dysfunction of granulosa cells and follicular development stagnation of PCOS.
