Role of high-throughput sequencing technology:a key tool in resolving forensic challenges in biological sample identification
10.13618/j.issn.1001-5728.2024.06.017
- VernacularTitle:高通量测序技术:解决法医学疑难生物检材鉴定的关键工具
- Author:
Anqi CHEN
1
;
Suhua ZHANG
1
Author Information
1. 复旦大学法庭科学研究院,上海 200032
- Publication Type:Journal Article
- Keywords:
Forensic genetics;
Challenging DNA samples;
Next generation sequencing;
Third generation sequencing;
Genotyping
- From:
Chinese Journal of Forensic Medicine
2024;39(6):730-740
- CountryChina
- Language:Chinese
-
Abstract:
Trace,degraded and mixed DNA evidence have been considered as the challengs in forensic genetics(or forence DNA)identification.Conventional short tandem repeat(STR)typing method based on capillary electrophoresis(CE)plays a role for DNA typing.However,the effectiveness is limited due to the restricted number of genetic markers that can be detected when dealing with complex and difficult evidence.With the continuous development of biotechnology,more and more genetic makers play roles in forensic application.They are using the high-throughput sequencing technologies which is represented by next generation sequencing(NGS)and third-generation sequencing techniques.And they also play an important role in the genotyping of difficult evidence.In this review,we summarized the primary detection methods and applications of high-throughput sequencing technologies in challenging biological samples,and discussed its future development trends as well as application prospects.
