Preparation and performance evaluation of immunochromatographic patch for rapid detection of interleukin-6 in human sweat
10.19745/j.1003-8868.2024232
- VernacularTitle:人体汗液中白介素-6快速检测免疫层析贴片的制备及性能评价
- Author:
Meng REN
1
;
Qiang HE
;
Ying-shuai LIU
;
Ping MA
;
Xu YANG
Author Information
1. 湖北科技学院医学部药学院,湖北咸宁 437100;湖北省智慧康养产业技术研究院,湖北咸宁 437100;西南大学材料与能源学院,重庆 400715
- Publication Type:Journal Article
- Keywords:
immunochromatography;
immunosandwich method;
human sweat;
IL-6;
immunochromatographic patch;
colloidal gold
- From:
Chinese Medical Equipment Journal
2024;45(12):32-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare an immunochromatographic patch for non-invasive and rapid detection of interleukin 6(IL-6)in human sweat and evaluate its performance.Methods Colloidal gold was prepared by the trisodium citrate reduction method,and optimization was carried out for the concentration of colloidal gold-labeled antibody in the reaction system and the pH value.Then an immunochromatographic patch was manufactured by immunosandwich method using colloidal gold as the chromogenic medium,which was divided into a hydrophilic region and a hydrophobic region.There were hydrophilic paper,sample pads,binding pads,nitrocellulose(NC)membranes and absorbent paper involved in the hydrophilic region,and hydrophobic paper,hydrophobic skin-fit adhesive,transparent film and polyvinyl chloride(PVC)backing plate in the hydrophobic region.The prepared immunochromatographic patch had its sensitivity and precision evaluated by detecting the 1L-6 in phosphate buffered saline(PBS),artificial sweat samples and real sweat samples,and its specificity by testing IL-6,TNF-a,SARS-CoV-2 and blank PBS in real sweat samples.Results The prepared immunochromatographic patch proved its high sensitivity by rapidly detecting IL-6 in PBS,artificial sweat and real sweat samples,with the lowest detection limit being 20 pg/mL for IL-6;the immunochromatographic patch had no cross-reactivity with TNF-a and SARS-CoV-2,and showed high specificity.Conclusion The prepared immunochromatography patch realizes non-invasive and rapid detection of IL-6 in human sweat,and can be used for clinical laboratory examinations.[Chinese Medical Equipment Journal,2024,45(12):32-38]
