Complex Network Analysis of Strengthening the Spleen,Transforming Stasis and Removing Toxins Methods in the Treatment of Gastric Precancerous Lesions
- VernacularTitle:健脾化瘀解毒法治疗胃癌前病变的复杂网络分析
- Author:
JiaYi ZOU
1
;
WanYi LIN
;
JiaQi AI
;
JianSong FANG
;
Wei LIU
Author Information
- Publication Type:Journal Article
- Keywords: Gastric precancerous lesions; Strengthening the spleen; transforming stasis and removing toxins methods; Data mining; Network pharmacology; Molecular docking; Mechanism of action
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(8):2226-2243
- CountryChina
- Language:Chinese
- Abstract: Objective Based on data mining,network pharmacology,and molecular docking techniques,the medication patterns and underlying mechanisms of the therapeutic approach of trengthening the spleen,transforming stasis and removing toxins methods(JPHYJD)in treating gastric precancerous lesions(GPL)were analyzed.Methods Initially,data mining was conducted to extract relevant literature on the application of JPHYJD in GPL.An analytical network map was then constructed to get the identification of a representative drug set.In the network pharmacology phase.Active compounds and their targets were retrieved from TCMSP,while disease-related targets specific to GPL were sourced from Gene Cards,OMIM,and TTD.A"drug-disease"target intersection was mapped using bioinformatics platforms,and an"active compound-key target"network was constructed in Cytoscape.Protein-protein interaction(PPI)networks were obtained from the STRING database,and GO and KEGG enrichment analyses were performed to elucidate biological processes and pathways.Preliminary validation was conducted using molecular docking technology.Results 63 prescriptions were analyzed,with Baizhu-Ezhu-Danshen-Baihuashecao identified as the most representative drug set.85 active compounds and 590 predicted targets were discovered,intersecting with 4,726 disease-related targets,resulting in 412 key targets.GO and KEGG enrichment analyses yielded 3,541 enriched terms and 196 pathways,respectively.Key compounds,such as quercetin and alexandrin,targeted pivotal proteins like tumor protein 53(TP53),signal transducer and activator of transcription 3(STAT3),and epidermal growth factor receptor(EGFR)were implicated in regulating critical pathways like PI3K/AKT,exerting therapeutic effects.Molecular docking results demonstrated stable binding interactions between the core active compounds and their targets,reinforcing the predicted interactions.Conclusion This study,integrating data mining,network pharmacology,and molecular docking techniques,successfully elucidated the therapeutic patterns and underlying mechanisms of the JPHYJD approach in GPL treatment.
