Study of Sijunzi Decoction Preventing Disuse Atrophy and Its Mechanism on the Muscle Stem Cells/Myonuclear Domain Pathway
- VernacularTitle:四君子汤预防失用性肌萎缩的作用及其肌肉干细胞/肌核域途径的实验研究
- Author:
Dan MA
1
;
Linlin ZHANG
;
Zhongkang ZHU
;
Songnan WANG
;
Xudong LIU
;
Lingzhi WANG
;
Dehong SHAN
;
Rui YU
Author Information
- Publication Type:Journal Article
- Keywords: Sedentary behavior damaging muscle; Disuse atrophy; Sijunzi decoration; Muscle stem cell; Myonuclear domain
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(8):2142-2149
- CountryChina
- Language:Chinese
- Abstract: Objective To study the action of Sijunzi decoction preventing disuse atrophy and its mechanism on the pathway of muscle stem cell(MuSC)/myonuclear domain(MND)based on the theory of sedentary behavior damaging muscle.Methods 40 male rats were randomly divided into the normal group,tail hanging group(model group),tail hanging+exercise group(exercise group),tail hanging+distilled water group(DW group),tail hanging+sijunzi decoction(Chinese medicine(CM)group);grip strength of limbs was measured by Grip Strength Meter for rats and mice;the wet weight of quadriceps femoris(QF)was measured by electronic scales;concentrations of actin and myosin were detected by ELISA;the cross section area(CSA)and myonuclear numbers were detected with Image Pro 6.0,and then MNDs were calculated;the expressions of pair box gene 7(Pax7)and myogenic differentiation antigen(MyoD)were detected by immunofluorescence.Results Compared with those in the normal group,grip strength,QF wet weight,the myosin concentration,the CSA and MND in the model and DW groups were all decreased,the numbers of Pax7 were increased significantly;compared with those in the model and distilled water groups,grip strength,QF wet weight,the myosin level,CSA and myonuclear numbers in the exercise group and CM groups were increased,and the numbers of Pax7 and MyoD positive cells were increased,markedly,however the number of Pax7 positive cells in the exercise group was more than that in the CM group significantly.Conclusion Sijunzi decoction can prevent disuse atrophy and its possible mechanism is related to the activation the MuSC/MND pathway.
