Effects of icariin on myocardial cell apoptosis and TGF-β1/Smad3 signaling pathway in rats with septic myocardial damage
10.12007/j.issn.0258-4646.2025.08.005
- VernacularTitle:淫羊藿苷对脓毒症心肌损伤大鼠心肌细胞凋亡和TGF-β1/Smad3信号通路的影响
- Author:
Xi ZHANG
1
;
Meng ZHANG
;
Jiang LI
;
Chengyang XU
Author Information
1. 郑州铁路职业技术学院河南省天然药物提取和医疗技术应用工程研究中心,郑州 450000
- Publication Type:Journal Article
- Keywords:
icariin;
sepsis;
myocardial damage;
apoptosis;
TGF-β1/Smad3 signaling pathway
- From:
Journal of China Medical University
2025;54(8):697-701,708
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of icariin on myocardial cell apoptosis and transforming growth factor-β1(TGF-β1)/Smad3 signaling pathway in rats with septic myocardial damage.Methods A septic myocardial damage model was established in SD rats via intraperitoneal injection of lipopolysaccharide.The rats with septic myocardial damage were randomly divided into model,low-dose icariin,high-dose icariin,and high-dose icariin+SRI-011381 groups(n=10 per group).An additional 10 rats that received intraperi-toneal injection of an equal volume of normal saline served as the control group.The left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD),left ventricular ejection fraction(LVEF),and left ventricular systolic pressure(LVSP)were detected using ultrasound instrument.Hematoxylin and eosin(HE)staining was used to detect the morphology of myocardial tissue.Trans-mission electron microscopy was used to measure the ultrastructure of myocardial tissue.The enzyme-linked immunosorbent assay was used to detect the serum markers of myocardial damage including cardiac troponin T(cTnT)and creatine kinase isoenzyme-MB(CK-MB)and inflammatory cytokines including interlenkin-6(IL-6)and tumor necrosis factor α(TNF-α).Western blotting was performed to assess the expression of TGF-β1/Smad3 pathway-related proteins in the myocardial tissue of rats in each group.Results Compared with the control group,LVEDD,LVESD,myocardial pathological score,myocardial cell apoptosis rate,the cTnT,CK-MB,IL-6,and TNF-α levels,TGF-β1 protein expression,and p-Smad3/Smad3 in the model group were higher(P＜0.05),while LVEF and LVSP were lower(P＜0.05).Compared with the model group,the pathological indictors of rats in the low-and high-dose icariin groups were improved,and high-dose icariin had a stronger effect.SRI-011381 could weaken the improvement effect of icariin on various pathological indicators in rats with septic myocardial damage.Conclusion Icariin can inhibit the inflammatory response in rats with sepsis by blocking the activation of the TGF-β1/Smad3 signaling pathway,thereby reducing myocardial cell apoptosis and myocardial damage.
