Characteristics of systemic immune microenvironment of DSS-induced acute ulcerative colitis in mice revealed by Mass cytometry
10.3969/j.issn.1000-484X.2025.09.016
- VernacularTitle:基于质谱流式细胞术揭示DSS诱导的小鼠急性溃疡性结肠炎全身免疫微环境特征
- Author:
Zongjing LYU
1
;
Jing XUN
;
Xiaolin JIANG
;
Bin LIU
;
Zehan LIU
;
Xueliang WU
;
Aimin ZHANG
;
Yu WU
;
Xiangyang YU
;
Ximo WANG
;
Qi ZHANG
Author Information
1. 天津医科大学研究生院,天津 300070
- Publication Type:Journal Article
- Keywords:
Mass cytometry(CyTOF);
Ulcerative colitis(UC);
Dextran sodium sulfate(DSS);
Immune microenvironment
- From:
Chinese Journal of Immunology
2025;41(9):2145-2152,中插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the characteristics of systemic immune microenvironment during the progression of dextran sulfate sodium(DSS)-induced acute ulcerative colitis(UC)induced in mice by Mass cytometry(CyTOF).Methods:Male C57BL/6 mice were randomly divided into control group and model group.The control group was given normal drinking water for 15 d.The mouse in the model group were given 5%DSS in drinking water,which was changed to normal drinking water after 7 days.In the model group,peripheral blood was collected on days 4,9 and 15,respectively.CyTOF was used to detect the expressions of 33 immune cell markers and changes in cell subsets in peripheral blood of mice,and the characteristics of systemic immune microenvironment in mice with acute UC were analyzed.Results:The cluster analysis of 33 kinds of immune cell markers showed that CD45+cells in peripheral blood of mice with DSS induced acute UC were divided into 23 fine subgroups,among which the proportions of B cell subgroup,T cell subgroup and neutrophil subgroup showed significant changes.A further dimensional reduction cluster analysis of T cell subsets found significant differences in the composition and proportion of the 10 identified T cell subsets.Conclusion:The systemic immune micro-environment map of mice with acute UC induced by DSS has been successfully constructed,and heterogeneity has been found in the systemic immune microenvironment of mice with acute UC.The changes and activation degree of T cell subpopulations are closely re-lated to disease progression and inflammation level.The results of this study provide theoretical basis for assisting the diagnosis,moni-toring the risk,progression,treatment and prognosis of acute UC.
