Stellate ganglion block alleviates inflammatory response in acute peritonitis rats by regulating α7nAChR expression
10.3969/j.issn.1000-484X.2025.09.015
- VernacularTitle:星状神经节阻滞通过调控α7nAChR表达减轻急性腹膜炎大鼠的炎症反应
- Author:
Yu'ao QIN
1
;
Xuejun ZOU
1
;
Ge LIU
1
;
Peng YANG
1
;
Lin LI
1
Author Information
1. 三峡大学附属仁和医院麻醉科,宜昌 443000
- Publication Type:Journal Article
- Keywords:
Stellate ganglion block;
Acute peritonitis;
Inflammation;
Cholinergic anti-inflammatory pathway
- From:
Chinese Journal of Immunology
2025;41(9):2141-2144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of stellate ganglion block(SGB)on the inflammatory response in rats with acute peritonitis,and to explore whether the possible anti-inflammatory mechanism related to the activation of α7nAChR receptor-mediated cholinergic anti-inflammatory pathway.Methods:A total of 36 SD rats were randomly divided into three groups,with 12 rats in each group:sham operation group(S group),acute peritonitis group(AP group)and SGB treatment group(SGB group).2%acetic acid(1 ml/100 g)was intraperitoneally injected to prepare acute peritonitis rat model in AP group and SGB group,and corresponding dose of normal saline was injected into S group.Right cervical sympathetic nerve was separated but not ligated in AP group and S group,the right cervical sympathetic nerve was severed and ligated in SGB group.Abdominal aorta blood samples were collected 24 h after opera-tion,and serum levels of IL-1β,IL-6 and TNF-α were measured by ELISA,pathological changes of peritoneal tissue were observed by light microscope and expression of α7nAChR in peritoneal tissue was measured by Western blot.Results:Compared with S group,se-rum levels of IL-1β,IL-6 and TNF-α in AP and SGB groups were significantly increased(P<0.05).Serum levels of IL-1β,IL-6,TNF-α in SGB group were significantly lower than those in AP group(P<0.05).HE staining of peritoneal tissues in each group revealed that the S group maintained intact peritoneal structure with normal cellular arrangement,while the AP group exhibited disorganized archi-tecture with extensive inflammatory cell infiltration,frequent hemorrhage,and prominent necrotic cells.In contrast,the SGB group displayed only mild inflammatory infiltration with preserved cellular morphology and minimal hemorrhagic or necrotic changes.The ex-pression level of α7nAChR protein in SGB group was significantly higher than that in S group and AP group.Conclusion:SGB can re-duce the inflammatory response of acute peritonitis rats,and its mechanism may be related to the cholinergic anti-inflammatory path-way mediated by α7nAChR.
