Circ_0039411 Regulates Neutrophil Extracellular Traps Through MiR-423-5p/IL-8 to Promote the Invasion of Gastric Cancer Cells
10.11969/j.issn.1673-548X.2025.07.013
- VernacularTitle:circ_0039411通过miR-423-5p/IL-8促进中性粒细胞胞外诱捕网的形成以促进胃癌细胞的侵袭
- Author:
Qi XU
1
;
Jiaojiao NI
1
;
Jingjing LI
1
Author Information
1. 310000 杭州,浙江省肿瘤医院肝胆胰胃内科
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Circ_0039411;
MiR-423-5p;
IL-8;
Neutrophil extracellular traps
- From:
Journal of Medical Research
2025;54(7):66-71,90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of circ_0039411 on gastric cancer cells by regulating neutrophil extracellular traps(NETs)through miR-423-5p/interleukin-8(IL-8).Methods We used quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)to detect the expression levels of circ_0039411 and miR-423-5p in gastric cancer cell lines(HGC-27,SNU-16 and AGS)and human gastric mucosa cell(GSE-1).We selected HGC-27 to establish a circ_0039411 low expression model,and set up Experimental group(transfected circ_0039411 siRNA)and Blank control group(transfected control siRNA).We selected the cell of Experimental group to establish a miR-423-5p low expression model,and set up inhibitor group(transfected miR-423-5p in-hibitor)and NC group(transfected empty).We used phorbol 12-myristate 13-acetate(PMA)to induce the formation of NETs in vitro,and established a PMA group(induced by PMA)and a control group(not induced by PMA).We used Transwell chamber to construct a co-culture model of gastric cancer cells and neutrophils.We used CCK-8 to detect the activity of gastric cancer cells,scratch and Tran-swell to detect the migration and invasion ability of gastric cancer cells,enzyme linked immunosorbent assay(ELISA)to detect the expres-sion level of IL-8,and immunofluorescence to detect the formation of NETs.Results The expression levels of circ_0039411 and IL-8 in gastric cancer cells HGC-27,SNU-16 and AGS were higher than those of human gastric mucosa cells GSE-1,and the expression level of miR-423-5p was lower than that of GSE-1,and the differences were statistically significant(P＜0.05).Compared with the Blank control group,the activity,migration and invasion ability of cells and the secretion of IL-8 in the Experimental group were de-creased,and the differences were statistically significant(P＜0.05),and the formation of NETs was reduced.Compared with NC group,the cell activity,migration and invasion ability and secreted IL-8 of inhibitor group were increased,and the differences were statistically significant(P＜0.05),and the formation of NETs was promoted.Compared with the control group,the PMA group exhibited significantly higher activity,migration ability,and invasion ability in gastric cancer cells,with statistically significant differences(P＜0.05).Conclu-sion circ_0039411 can promote the formation of NETs through the miR-423-5p/IL-8 axis,and further promote the migration and in-vasion ability of gastric cancer cells.
