Effect of miR-147a Targeting INHBA-TGF-β1/Smads Signaling Pathway on Proliferation,Migration and Invasion of Cervical Cancer HeLa Cells
10.11969/j.issn.1673-548X.2025.08.014
- VernacularTitle:miR-147a靶向INHBA-TGF-β1/Smads信号通路对宫颈癌HeLa细胞增殖、迁移与侵袭的作用
- Author:
Chunyu ZHANG
1
;
Junkai ZOU
1
;
Qi ZHOU
1
Author Information
1. 830000 乌鲁木齐,新疆医科大学附属肿瘤医院妇外四科
- Publication Type:Journal Article
- Keywords:
miR-147a;
INHBA;
TGF-β1/Smads;
Cervical cancer
- From:
Journal of Medical Research
2025;54(8):80-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miR-147a targeting inhibin subunit beta A(INHBA)in transforming growth factor-β1(TGF-β1)/Smads signaling pathway in the invasion and metastasis of cervical cancer HeLa cells.Methods The miR-147a mimics,miR-147a inhibitors and their negative controls(mimicsNC and inhibitor NC)were transfected into HeLa cells and divid-ed into five groups:control group(blank control),mimics NC group,miR-147a mimics group,inhibitor NC group,and miR-147a in-hibitor group.The proliferation activity of HeLa cells after miR-147a intervention was detected by the MTT method,the invasion and me-tastasis abilities of HeLa cells after the intervention of miR-147a were detected by Transwell and cell scratch assay,the mRNA expres-sion changes of miR-147a and INHBA in different groups were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR),the relationship between miR-147a and the target gene INHBA was detected by dual-luciferase reporter gene assay,the protein expressions of TGF-β1,TGFBR1,Smad3,Smad2 and INHBA in different groups were detected by Western blot,and the ex-pression changes of TGF-β1 were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with blank control and negative control,miR-147a mimics inhibited cell proliferation,decreased cell invasion and migration ability,and decreased cell healing ability.miR-147a inhibitor promoted cell proliferation,increased invasion and migration ability,and improved cell healing abili-ty.In the miR-147a mimics group,miR-147a was significantly increased,and the target gene INHBA was significantly decreased(P＜0.05).In the miR-147a inhibitor group,miR-147a was significantly decreased,and the target gene INHBA was significantly in-creased(P＜0.05).The fluorescence intensity ratio of miR-147a mimics+WT group was significantly lower than that of mimics NC+WT group(P＜0.05).The expression levels of TGFBR1,TGF-β1,Smad3,Smad2 and INHBA in miR-147a mimics group were sig-nificantly decreased(P＜0.05).The expression levels of TGFBR1,TGF-β1,Smad3,Smad2,and INHBA in miR-147a inhibitor group were significantly increased(P＜0.05).Conclusion miR-147a targets the TGF-β1/Smads signaling pathway mediated by IN-HBA,which plays an important role in inhibiting the proliferation,invasion and migration of cervical cancer HeLa cells,and provides a potential molecular target and theoretical basis for the treatment of cervical cancer.
