Establishment of a visual detection method for SARS-CoV-2 based on Au@Pt nanozyme and magnetic beads
10.3969/j.issn.1002-2694.2025.00.082
- VernacularTitle:基于铂金纳米酶和磁珠的新冠病毒可视化检测方法的建立
- Author:
Bingnian WANG
1
;
Lei ZUO
;
Jichuang WANG
;
Yulin LI
;
Lei CHENG
;
Xiaojun WANG
;
WANG DAVID XUDONG
;
Yunlong WANG
Author Information
1. 新乡医学院河南省分子诊断与医学检验技术协同创新中心,新乡 453003;河南省生物工程技术研究中心,郑州 450000
- Publication Type:Journal Article
- Keywords:
Au@Pt nanoparticles;
magnetic beads;
SARS-CoV-2;
immunodetection
- From:
Chinese Journal of Zoonoses
2025;41(7):712-717,725
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at establishing a novel visual detection method for SARS-CoV-2 based on magnetic beads(MBs)enrichment and Au@Pt nanoparticles(Au@PtNPs)enzyme catalysis.According to the double-antibody sandwich method,MBs conjugated with SARS-CoV-2 antibody 1 enriched SARS-CoV-2 antigen in samples.After magnetic adsorption,separation,and washing,the enriched antigen reacted with Au@PtNPs labeled with SARS-CoV-2 antibody 2,thereby forming an immune com-plex.After another round of magnetic adsorption and washing,the color change was catalyzed by Au@PtNPs in the chromogenic solu-tion,and the results were determined through comparison of color changes:a blue color change indicated a positive result,whereas no obvious color change indicated a negative result.The sensitivity and specificity of this method were evaluated.This study successfully established a visual detection method based on MBs enrichment and Au@PtNPs enzyme catalysis for targeting SARS-CoV-2 antigens.The method was able to detect SARS-CoV-2 antigens within 30 minutes and SARS-CoV-2nucleocapsid protein samples with antigen concentrations above 3.125 ng/mL.The method showed no cross-reactivity with seasonal influenza(H1N1)or influenza B virus nu-cleoprotein.The visual detection method established in this study,based on Au@PtNPs enzyme catalysis and MBs enrichment,has high specificity and sensitivity,is simple and quick to operate,and provides a new approach for rapid viral detection.
