Molecular epidemiological investigation of Babesia infection in small mammals in the Jinsha River Basin,Yunnan Province
10.3969/j.issn.1002-2694.2025.00.090
- VernacularTitle:云南省金沙江流域小型兽类感染巴贝虫的分子流行病学调查
- Author:
Fan WANG
1
;
Yun ZHANG
;
Zongti SHAO
;
Yuqiong LI
;
Ennian PU
;
Zhihai HE
;
Mingguo YAO
;
Shuangshuang BIE
;
Jiafu JIANG
;
Chunhong DU
Author Information
1. 云南省地方病防治所,云南省自然疫源性疾病防控重点实验室,大理 671000;北京大学首钢医院,北京 100041
- Publication Type:Journal Article
- Keywords:
Yunnan Province;
Jinsha River basin;
small mammals;
Babesia;
investigation
- From:
Chinese Journal of Zoonoses
2025;41(7):767-774
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at understanding the Babesia species makeup and distribution in small mammals in Jinsha River Basin of Yunnan Province,and the Babesia carriage status in small mammals in this area,to provide a scientific basis for the preven-tion and control of Babesia disease.A total of 1 493 small mammals belonging to 5 orders,10 families,25 genera,and 54 species were captured from 10 counties(cities)in the Jinsha River Basin of Yunnan Province in various agricultural and forest environments.DNA was extracted from liver and tick tissues,and 150 bp fragments of Babesia 18S rRNA were detected through molecular biological methods.The positive samples showed amplification of a 1 600 bp target fragment of 18S rRNA.Species characteristics were assessed through sequence comparison and phylogenetic analysis.A total of 14 small mammals infected with Babesia were detected in six coun-ties(cities)of Jinsha River Basin,Yunnan Province,with a positivity rate of 0.93%(14/1 493).The Otsu and Kobe types of Babesia voles were analyzed,and their sequences were compared with the sequences from human Babesia cases with high similarity and close evolutionary relationships.The positivity rates were 2.34%(3/128)in Qiaojia County,2.06%(2/97)in Yongshan County,1.88%(4/213)in Yuanmou County,1.03%(3/291)in Deqin County,0.95%(1/105)in Shangri-La City,and 0.78%(1/128)in Shuifu County.The positive small mammals belonged to one order,two families,six genera,and the following eight species:P.leucurus 5.56%(1/18),R.brunneusculus 3.36%(4/119),M.minutus 3.33%(1/30),E.custos 2.94%(1/34),N.confucianus 2.65%(3/113),N.fulvescens 2.35%(2/85),A.latronum 1.16%(1/86),and A.draco 0.98%(1/102).The detection of Babesia in M.minutus was re-poorted first time.Small animals infected with Babesia were detected in all three habitats and altitudes,and higher infection rates were observed in forest regions between 1 500 and 2 500 meters and high-altitude residential areas.Babesia infection was found in many small mammals in several counties(cities)along Jinsha River in Yunnan Province,and the epidemic status of Babesia in these areas warrants attention.
