Survey of coronaviruses carried by bats in Qinghua Cave,Yunnan Province,China,and establishment of a quantitative viral detection method
10.3969/j.issn.1002-2694.2025.00.080
- VernacularTitle:云南省清华洞蝙蝠携带的冠状病毒调查与病毒定量检测方法的建立
- Author:
Wei KONG
1
;
Peiyu HAN
1
;
Ze YANG
1
;
Junying ZHAO
1
;
Yi TANG
1
;
Jiawei TIAN
1
;
Fenhui XU
1
;
Lidong ZONG
1
;
Yunzhi ZAHNG
1
Author Information
1. 大理大学公共卫生学院,大理大学预防医学研究所,云南省滇西抗病原植物资源筛选研究重点实验室,云南省高校人兽共患病跨境防控与检疫重点实验室,大理 671000
- Publication Type:Journal Article
- Keywords:
bat;
betacoronavirus;
reverse transcription-polymerase chain reaction;
quantitative reverse transcription polymerase chainreaction(qRT-PCR)
- From:
Chinese Journal of Zoonoses
2025;41(7):704-711
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to qualitatively and quantitatively detect coronavirus(CoV)in the feces of bats from Qinghua Cave,Yunnan Province,China.CoV was qualitatively tested with reverse transcription polymerase chain reaction(RT-PCR),and homology and genetic evolution were analyzed with bioinformatics software.The established reverse transcription real-time fluores-cence quantitative PCR(qRT-PCR)method was applied to CoV quantification in bat feces.The positivity rate of CoV in 306 fecal samples collected from the fulvous fruit bat(Rousettus leschenaultia)was 7.8%(24/306)according to RT-PCR.All 24 strains of CoV belonged to β-CoV,and showed a similarity of 86.8%-100.0%at the nucleotide level and 95.2%-100.0%at the amino acid level,with respect to other β-CoV sequences in the NCBI database.The positivity rate of CoV was 18.6%(57/306)according to qRT-PCR,a value higher than that according to RT-PCR(χ2=25.3,P<0.05).The mean β-CoV load was 1.3×103 copies/μL.In conclusion,the bats in Qinghua Cave,Yunnan Province,carried CoV belonging to β-CoV.The established qRT-PCR method achieved good sensitiv-ity,accuracy,reproducibility,and a higher detection rate than that of RT-PCR,and can be used for rapid detection of β-CoV in bats.
