Genomic evolutionary characteristics of 69 Legionella pneumophila strains in Fujian Province
10.3969/j.issn.1002-2694.2025.00.084
- VernacularTitle:福建省69株嗜肺军团菌全基因组进化特征分析
- Author:
Junjie MAO
1
;
Rui JIN
;
Xuechen LIANG
;
Yuhang DENG
;
Yadong GAO
;
Quwen LI
Author Information
1. 福建医科大学公共卫生学院,福州 350122
- Publication Type:Journal Article
- Keywords:
Legionella pneumophila;
whole genome sequencing;
antibiotic resistant gene;
mobile genetic elements
- From:
Chinese Journal of Zoonoses
2025;41(7):691-696
- CountryChina
- Language:Chinese
-
Abstract:
Legionella pneumophila is an important pathogen responsible for legionellosis.Understanding the genetic diversity,evo-lutionary traits,and potential pathogenicity of L.pneumophila in Fujian Province is crucial for the implementation of effective public health interventions.This study was aimed at investigating these aspects through the analysis of environmental isolates collected from 2019 to 2024.A total of 69 environmental isolates of L.pneumophila were collected,and serotypes were determined with agglutination methods.The predominant serotypes were LP1 and LP5,identified in 23 isolates(33.33%)and 15 isolates(18.84%),respectively.Whole-genome sequencing was conducted to assess the genomic characteristics of these isolates.The genomic sizes ranged from 3.26 Mb to 3.81 Mb,and the GC content ranged from 38.0%to 38.3%.Sequence type(ST)1 880 emerged as the dominant strain among the isolates.All isolates carried several virulence-associated genes,including mip,iraA,htpB,LPG_RS03055,and lspM,thus indicat-ing strong potential for pathogenicity.The prevalence of the multidrug resistance genes lpeA and lpeB was 23.2%,thereby suggesting a concerning level of antibiotic resistance.Various mobile genetic elements,such as genomic islands,insertion sequences,phages,and CRISPR/Cas systems,were also detected.In conclusion,our findings emphasize the importance of continued research and surveil-lance of L.pneumophila,particularly the ST1880 clone,to gain a deeper understanding of its transmission mechanisms and to imple-ment control measures effectively.These insights should aid in enhancing public health responses to legionellosis in Fujian Province.
