Mechanism of action of Sterculiae Lychnophorae Semen against PM2.5-induced acute lung injury based on network pharmacology,molecular docking and experimental validation
- VernacularTitle:基于网络药理学、分子对接及实验验证探讨胖大海抗PM2.5诱导急性肺损伤的作用机制
- Author:
Fan ZHANG
1
;
Yi-fan DU
;
Xiao-shu DENG
;
Zu-feng ZHANG
;
Xian-lei HAN
;
Wei TIAN
;
Xiu-mei LI
;
Mian CHEN
;
Fei LIU
;
Nan WANG
Author Information
- Publication Type:Journal Article
- Keywords: acute lung injury; Sterculiae Lychnophorae Semen; PM2.5; network pharmacology; molecular docking; MAPK
- From: Chinese Pharmacological Bulletin 2025;41(12):2362-2369
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the anti-acute lung injury(ALI)effect of Sterculiae Lychnophorae Semen(SLS)and its mechanism.Methods The main ac-tive components of SLS and their core targets and path-ways of action against ALI were obtained by network pharmacology methods.Subsequently,molecular doc-king technology and in vitro cellular experiments were applied for validation.Results A total of 19 core tar-gets were obtained,including HSP90AA1,CASP3,TNF,MAPK8 and MAPK14.The mechanisms may in-volve signaling pathways such as cancer,PI3K/Akt and MAPK.Molecular docking confirmed that the key targets of SLS formed a better binding activity with the relevant active ingredients.The in vitro results showed that SLS was able to protect the PM2.5-contaminated BEAS-2B cells,inhibit their NO,IL-1β and TNF-αlevels,and reduce the expression of p-p38 MAPK and p-JNK proteins.Conclusions The study successfully predicts the active ingredients,targets and signaling pathways of SLS against ALI,and in vitro experiments demonstrate that SLS might protect BEAS-2B cells from PM2.5 stimulus-induced inflammation and apoptosis by inhibiting the over-activation of p38 MAPK and JNK signaling pathways.
