Tanshinone ⅡA regulates cuproptosis-related proteins and affects lipopolysaccharide-induced inflammatory damage in H9c2 cardiomyocytes
- VernacularTitle:丹参酮ⅡA调控铜死亡相关蛋白影响脂多糖诱导的H9c2心肌细胞炎症损伤
- Author:
Zi-hao XIE
1
;
Hong-chun HU
1
;
Hong-juan YAN
1
;
Hua-ying WU
1
Author Information
- Publication Type:Journal Article
- Keywords: tanshinone ⅡA; lipopolysaccharide; H9c2 cardiomyocytes; inflammation; copper ion; cu-proptosis
- From: Chinese Pharmacological Bulletin 2025;41(12):2275-2282
- CountryChina
- Language:Chinese
- Abstract: Aim To establish a lipopolysaccharide(LPS)-induced inflammatory injury model in H9c2 cardiomyocytes,and to investigate the anti-inflammato-ry effects of tanshinone ⅡA(Tan ⅡA)and its influ-ence on cuproptosis.Methods Cell viability was de-tected by CCK-8 method.The mRNA expression levels of tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),and interleukin-6(IL-6)were meas-ured by RT-qPCR.Intracellular copper ion levels were detected via colorimetry.The protein expression levels of cuproptosis-related markers(CTR1,FDX1,DLAT,PD H α1)were determined using Western blot and im-munofluorescence techniques.Results Tan ⅡA sig-nificantly inhibited LPS-induced upregulation of in-flammatory cytokines(TNF-α,IL-1β,and IL-6)and Cu+accumulation in H9c2 cardiomyocytes.Moreover,Tan ⅡA reversed the exacerbated inflammatory re-sponse and elevated Cu+levels induced by the combi-nation of LPS and the copper ionophore Elesclomol-Cu(Ⅱ).Additionally,Tan ⅡA markedly downregulated the protein expression of CTR1,FDX1,DLAT,and PDHα1.Conclusion Tan ⅡA alleviates LPS-in-duced inflammatory injury in H9c2 cardiomyocytes by regulating intracellular Cu+levels and the expression of cuproptosis-related proteins.
