Application of magnetic bead-based liquid chromatography-tandem mass spectrometry(LC-MS/MS)for detection of cate-cholamines in human plasma
10.13602/j.cnki.jcls.2024.12.01
- VernacularTitle:磁珠法结合液相色谱串联质谱技术应用于人血浆儿茶酚胺检测
- Author:
Lei HUA
1
;
Jingxian HAN
;
Wenchao HAN
;
Siqi ZHANG
Author Information
1. 郑州市第七人民医院河南省心脏重构与移植重点实验室,郑州 450016
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
catecholamine metabolites;
magnetic bead-based method;
solid-phase extraction(SPE)
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(12):881-886
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of magnetic bead-based liquid chromatography-tandem mass spectrometry(LC-MS/MS)in extracting and detecting catecholamine metabolites in plasma.Methods The catecholamine metabolites,i.e.,dopa-mine(DA),norepinephrine(NE),epinephrine(E),3-methoxytyramine(3-MT),normetanephrine(NMN),and metanephrine(MN),were extracted from plasma using magnetic bead-based LC-MS/MS.The performance of the method was validated by assessing linear range,detection limit,precision,and matrix effects.Clinical plasma samples(n=81)were collected,and the results of mag-netic bead-based extraction were compared with traditional solid-phase extraction(SPE)using LC-MS/MS and the statistical analysis were conducted for consistency of the two extraction methods.Results The linear correlation coefficients of DA,NE,E,3-MT,NMN and MN extracted using magnetic beads were all greater than 0.99,and the limits of quantification were determined to be 10.41,80.2,4.55,3.89,11.2 and 1.12 pg/mL,and the limits of detection were established as 2.75,26.5,1.5,1.35,3.1 and 0.34 pg/mL,re-spectively.The intra-and inter-day precision expressed as coefficient of variation(CV)were both within 15％.The relative recovery rates ranged from 100.54％to 102.21％for DA,95.78％to 101.85％for NE,95.56％to 99.09％for E,95％to 102.72％for 3-MT,94.97％to 100.19％for NMN,and 94.77％to 100.65％for MN with the range of all the relative deviations within±15％.All the rela-tive matrix effects were within the range of 85％to 115％.The comparisons of magnetic bead extraction with SPE for DA,NE,E,3-MT,NMN and MN showed the relative percent deviations of 0.93％,0.99％,0.95％,0.92％,0.97％and 1.02％,respectively,in-dicating fine consistency between the two extracting methods.Conclusion The magnetic bead-based method in extracting cate-cholamine metabolites from plasma was demonstrated to possess excellent analytical performance,rapid and simple operation,and could enhance the automation degree and throughput in the process of clinical testing,showing promising application prospects.
