The effect of resistance exercise regulation of Piezo1 on myoblasts in skeletal muscles in elderly mice
10.3969/j.issn.1006-5725.2025.23.002
- VernacularTitle:抗阻运动调节Piezo1对老年小鼠骨骼肌成肌细胞的影响
- Author:
Rong LIN
1
;
Gang LIU
1
Author Information
1. 南方医科大学南方医院康复理疗科(广东 广州 510515)
- Publication Type:Journal Article
- Keywords:
annual muscular atrophy;
resistance exercise;
Piezo1;
aging;
skeletal muscles
- From:
The Journal of Practical Medicine
2025;41(23):3638-3644
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects of resistance exercise on Piezo1 in myoblasts within skeletal muscles of aged mice.Methods In animal experiments,thirty 20-month-old male C57BL/6 mice were randomly assigned to one of three groups:an aging control group,an aging exercise group,and an aging exercise inhibitor group.Additionally,ten 3-month-old young mice served as a young control group.The aging exercise group underwent 8 weeks of resistance exercise training,whereas the aging exercise inhibitor group received both 8 weeks of resistance exercise and intraperitoneal injections of GsMTx4.Exercise capacity was evaluated using grip strength tests and rotarod performance assays.Skeletal muscle cross-sectional area was assessed via hematoxylin-eosin staining,fibrosis was examined using Sirius red staining,and the proportion of p16-positive cells was determined by immunohistochemistry.In cellular experiments,senescence in myoblasts was induced with D-galactose(D-gal),followed by activation of Piezo1 using Yoda1.The percentage of senescent cells was measured by SA-β-Gal staining,while protein expression levels of senescence markers(p16 and p21)and atrophy markers(Atrogin-1 and MuRF1)were quantified by Western blot analysis.Cell immunofluorescence staining was employed to assess the average fluorescence intensity of p21.Results(1)Compared with the aging control group,the aging+exercise group exhibited significant improvements in exercise capacity,quadriceps femoris mass index,skeletal muscle cross-sectional area,reduction in skeletal muscle fibrosis,and a lower p16-positive cell percentage(P<0.05);(2)These beneficial effects of exercise on aged skeletal muscle were attenuated following treatment with a Piezo1 inhibitor(P<0.05);(3)In vitro experiments demonstrated that activation of Piezo1 reduced the SA-β-Gal positive cell rate in myoblasts,downregulated the expression of senescence markers(p16 and p21)and atrophy-related markers(Atrogin-1 and MuRF1),and decreased the average fluorescence intensity of p21(P<0.05).Conclusion Resistance exercise suppresses myocyte aging through activation of Piezo1,thereby ameliorating age-related muscle atrophy in the elderly.
