Exploring the Effect of Enema Therapy for Constraining Sores and Promoting Tissue Regeneration on the Colonic Mucosal Barrier in Rats with Ulcerative Colitis Based on the JAK/STAT Signaling Pathway
10.11842/wst.20231201004
- VernacularTitle:基于JAK/STAT信号通路探讨"敛疮生肌方"灌肠疗法对UC模型大鼠结肠黏膜屏障的作用
- Author:
Zhuhua LIU
1
;
Caifeng ZHANG
;
Julian HUANG
;
Wendi XUE
Author Information
1. 山西中医药大学附属医院 太原 030024
- Publication Type:Journal Article
- Keywords:
Conquering sore and promoting muscle enema;
Ulcerative colitis;
Mucosal barrier;
JAK2/STAT3 signaling pathway
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2024;26(11):2980-2987
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the regulation of JAK/STAT signaling pathway by the therapy of astringent ulcer and muscle inducing enema on colon injury and mucosal barrier in ulcerative colitis(UC)model rats.Methods A rat model of ulcerative colitis was established using 2,4,6-trinitrobenzenesulfonic acid/ethanol method.The model was randomly divided into a normal control group(without modeling),a model group,a positive drug group(mesalazine sustained-release granules 0.42 g·kg-1),a high dose of Lianchuang Shengji Formula(enema 10.5 g·kg-1),a medium dose(enema 5.25 g·kg-1),and a low dose group(enema 2.62 g·kg-1).Compare the fecal occult blood,degree of colonic mucosal damage,and pathological changes of rats in each group,and detect the expression of JAK2,STAT3 proteins,and their phosphorylated proteins in colon tissue using Western blot method.Results The results showed that the mesalazine group,the medium and high-dose groups of Lianchuang Shengji Formula could increase the body mass of rats,reduce fecal occult blood score,improve colon damage,and achieve pathological results;The mesalazine group and the low,medium,and high dose groups of Lianchuang Shengji can downregulate the expression of p-JAK2 and p-STAT3 proteins(compared with the model group,P＜0.05),with the high-dose group being the most significant(P＜0.01).The apoptosis rate of colon tissue significantly decreased(P＜0.05).Conclusion The enema therapy of astringent ulcer and promoting muscle growth can significantly improve the pathological scores of bloody stools and colon injury in rats with ulcerative colitis.The mechanism may be related to the inhibition of JAK2 and STAT3 phosphorylation protein expression,and further inhibition of the JAK/STAT signaling pathway.
