Protective effect of basic alkaline ceramidase 1 in ulcerative colitis
10.3969/j.issn.1006-5725.2025.01.002
- VernacularTitle:碱性神经酰胺酶1在硫酸钠葡聚糖诱导小鼠溃疡性结肠炎中的保护作用
- Author:
Rongmao HE
1
;
Zeyang FANG
;
Yunyun ZHANG
;
Youliang WU
;
Shixiu LIANG
;
Siqi WANG
Author Information
1. 广州医科大学附属第一医院消化内科(广东 广州 510120)
- Publication Type:Journal Article
- Keywords:
alkaline ceramidase 1;
ulcerative colitis;
mechanical barrier;
mucus barrier;
inflam-matory injury
- From:
The Journal of Practical Medicine
2025;41(1):7-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role and influence of basic Alkaline ceramidase 1 on mucosal barrier and immune regulation in ulcerative colitis(UC).Methods Acer1 knockout mice(Acer1 KO)were constructed,and the UC model was induced by continuous drinking water of sodium dextran sulfate(DSS)for 7 days,and the severity of symptoms of UC disease in C57 and Acer1 KO mice was compared.The expression levels of intestinal mucosal barriers(ZO-1,Occludin,Claudin,JAMA)were detected by RT-PCR and immunohistochemistry.Systemic immune response levels(IL-1b、IL-6、IL-23、IL-17、IL-10,and TNF-a)were assessed by ELISA and intestinal inflammatory infiltration levels(TNF-a、IL-1b、IL-6、IL-17、IL-21,etc.)were assessed by RT-PCR.Results There was no significant difference between C57 and Acer1 KO mice in free drinking water.In the DSS induced UC model,compared with C57 mice,the survival rate of Acer1 KO mice decreased,the weight decreased significantly,the mechanical barrier and mucus barrier protein expression levels decreased significantly,the intes-tinal epithelial barrier was seriously damaged,the inflammatory response was strong,and the cytokine infiltration was obvious,with statistical differences(P<0.05).Conclusion Acer1 can inhibit inflammatory infiltration by maintaining the integrity of intestinal mucosal barrier,preventing endotoxin,and delaying the progression of UC.
