Exploring the Effect of Ganmai Dazhao Tang on Intestinal Inflammation in ADHD Model Rats Based on Network Pharmacology
- VernacularTitle:基于网络药理学探讨甘麦大枣汤对ADHD模型大鼠肠道炎症的影响
- Author:
Chenhao XUE
1
;
Yunqian XIAN
;
Dan TANG
;
Jiacheng WANG
;
Yamin HE
;
Jingjing YANG
;
Shaofang GAO
Author Information
- Publication Type:Journal Article
- Keywords: Ganmai Dazhao Tang; Network pharmacology; ADHD; Inflammation of the intestines
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(11):2968-2979
- CountryChina
- Language:Chinese
- Abstract: Objective Based on network pharmacology,protein mechanism,and experimental validation,this study aims to explore the role of Ganmai Dazao Tang in treating intestinal inflammation in children with attention deficit hyperactivity disorder(ADHD)model rats.Methods Screening the relevant targets and pathways for the effect of Ganmai Dazhao Tang on intestinal inflammation in ADHD model rats using network pharmacology methods,and verifying the affinity of the main components and targets through molecular docking.Forty spontaneously hypertensive rats(SHR)were randomly divided into model group,methylphenidate group,and low,medium,and high dose groups of Ganmai Dazao Tang,with 8 rats in each group.Additionally,8 Wistar Kyoto rats(WKY)were set as the normal group.Rats in each group were treated daily by gavage for 3 weeks.Observing the pathological condition of the small intestine using HE staining technology,and detecting interleukin-1 using enzyme-linked immunosorbent assay(ELISA)and Western blot methods β(IL-1β),tumor necrosis factor α(TNF-α),Expression of the oncogene Fos(FOS).Results Network pharmacology screened 117 chemical components and predicted 248 effective targets.Molecular docking results showed that Isovitexin β-Ingredients such as beta carotene,kaempferol,luteolin,and naringenin have strong affinity with targets such as AKT1,FOS,VEGFA,and TNF.Animal test results show that Ganmai Dazhao Tang can improve the core symptoms of ADHD,protect normal intestinal function and status,and downregulate related IL-1 β,TNF-α,FOS factor content.Conclusion Ganmai Dazao Tang can alleviate the core symptoms of ADHD in SHR rats by protecting normal intestinal function and downregulating inflammatory factors.
